• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Plant Breeding and Genetics >Effect of Dimethyl Sulphate on the Growth, Induction of Mutations and Their Identification by Peroxidase Isozyme in Tecoma stans
【24h】

Effect of Dimethyl Sulphate on the Growth, Induction of Mutations and Their Identification by Peroxidase Isozyme in Tecoma stans

机译：硫酸二甲酯对Tecoma stans生长，诱导突变及其过氧化物酶同工酶鉴定的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Tecoma stansls an important plant as flowering perennial shrub and herbal medicine. Methodology: Seeds were soaked in Dimethyl Sulphate Solution (DMS) at the concentrations of 0,200,400,600,800 and 1000 ppm for 15 h to induce genetic variability. Results: The concentration of 600 ppm had stimulative effect on all studied traits of vegetative growth in the 1st generation only. While, the adverse effect was occurred in the M2 generation. The concentrations of 200 and 1000 ppm increased significantly the number of florets per inflorescence in both generations. Most treatments produced changes in the leaf form in both generations. All valuable mutants were produced in the 2nd generation. The treatments of 400 and 600 ppm produced plants withlobed pinnately margins of the leaflets (M5). Florets without the orange strip in the throat were found after the concentration of 800 ppm. Florets with four petals were found using 800 ppm and plant having large number of florets in its inflorescence was also found. Dwarfed plant produced using 600 ppm. Thirteen plants with large leaves were obtained after 1000 ppm treatment (M3). Different profile among mutants was found using peroxidase isozyme. Similarity values indicated that, all mutants differedgenetically from control with different genetic distances. The dendrogram tree classified the mutants to two clusters. Mutants 3 and 5 were grouped in cluster A and the other mutants and control were grouped in cluster B. Conclusion: Dimethyl sulphate is a powerful mutagens for inducing genetic variability and valuable mutants in Tecoma stans, which can be vegetatively propagated as new cultivar.
机译：背景：Tecoma是多年生开花灌木和草药的重要植物。方法：将种子浸入浓度为0,200,400,600,800和1000 ppm的硫酸二甲酯溶液（DMS）中15 h，以诱导遗传变异。结果：600 ppm的浓度仅对第一代营养生长的所有研究性状都有刺激作用。同时，不良反应发生在M2代中。在两代中，200和1000 ppm的浓度显着增加了每花序的小花数量。大多数处理在两代中都改变了叶的形态。所有有价值的突变体均产生于第二代。处理400和600 ppm的植物产生的叶具小叶羽状边缘（M5）。浓度为800 ppm后，发现喉咙中没有橙色条纹的小花。使用800ppm发现了具有四个花瓣的小花，并且还发现了在其花序中具有大量小花的植物。使用600 ppm生产的矮化植物。 1000 ppm处理（M3）后，获得了13片大叶植物。使用过氧化物酶同工酶发现了突变体之间的差异。相似值表明，所有突变体在遗传距离上均与对照突变。树状图将突变体分为两个簇。突变体3和5归为A类，其他突变体和对照归为B类。结论：硫酸二甲酯是一种强大的诱变剂，可诱导特科马斯坦的遗传变异和有价值的突变体，可以作为新品种在营养上繁殖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号