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首页> 外文期刊>Journal of environmental science and health, Part A. Toxic/hazardous substances & environmental engineering >Endocrine disruptive effects of cadmium on steroidogenesis: Human adrenocortical carcinoma cell line NCI-H295R as a cellular model for reproductive toxicity testing
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Endocrine disruptive effects of cadmium on steroidogenesis: Human adrenocortical carcinoma cell line NCI-H295R as a cellular model for reproductive toxicity testing

机译：镉对类固醇生成的内分泌干扰作用：人肾上腺皮质癌细胞系 NCI-H295R 作为生殖毒性测试的细胞模型

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摘要

Cadmium (Cd) is a known endocrine disruptor with the ability to affect the production of hormones involved in the regulation of reproductive processes. In this study human adrenocortical carcinoma cell line NCI-H295R was used as an in vitro biological model to study the effect of cadmium (CdCl2) on steroidogenesis. The cell cultures were exposed to different concentrations of CdCl2 (1.90, 3.90, 7.80, 15.60, 31.20 and 62.50 mu M) and compared to control (medium without CdCl2). Cell viability was measured by the metabolic activity (MTT) assay for estimation of mitochondria structural integrity. Quantification of sexual steroid production directly from aliquots of the medium was performed by enzyme linked immunosorbent assay (ELISA). Following 48h culture of the cells in the presence of CdCl2 a concentration-dependent depletion in progesterone production was observed at the lower concentrations of CdCl2. The lowest amount of progesterone was significantly detected in groups with the higher doses (>= 31.20 mu M) of CdCl2, which elicited significant (P 75) up to 7.80 mu M of CdCl2 and significantly (P < 0.01) decreased at 15.60 mu M and higher concentrations of CdCl2. These results suggest that cadmium has endocrine disruptive effects on sexual steroid synthesis even at very low concentrations.
机译：镉（Cd）是一种已知的内分泌干扰物，能够影响参与生殖过程调节的激素的产生。本研究以人肾上腺皮质癌细胞系NCI-H295R为体外生物学模型，研究镉（CdCl2）对类固醇生成的影响。将细胞培养物暴露于不同浓度的CdCl 2（1.90、3.90、7.80、15.60、31.20和62.50μM）中，并与对照（不含CdCl 2的培养基）进行比较。通过代谢活性 （MTT） 测定法测量细胞活力，以估计线粒体结构完整性。通过酶联免疫吸附测定（ELISA）对直接从培养基等分试样中产生的性类固醇进行定量。在CdCl 2存在下培养细胞48小时后，在较低浓度的CdCl 2下观察到黄体酮产生的浓度依赖性耗竭。在CdCl2剂量较高（>=31.20μ M）的组中，黄体酮含量最低，也引起显著（P75%），在15.60μM和更高的CdCl2浓度下显著降低（P<0.01）。这些结果表明，即使在非常低的浓度下，镉对性类固醇合成也有内分泌破坏作用。

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