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首页> 外文期刊>Vaccine >Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV
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Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV

机译：结合 MVA 载体和植物来源的 VP2 作为对抗 IBDV 策略的同源和异源初免-加强免疫的比较

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Different immunogens such as subunit, DNA or live viral-vectored vaccines against Infectious Bursal Disease virus (IBDV) have been evaluated in the last years. However, the heterologous prime-boost approach using recombinant modified vaccinia Ankara virus (rMVA), which has shown promising results in both mammals and chickens, has not been tried against this pathogen yet. IBD is a highly contagious and immunosuppressive disease of poultry that affects mainly young chicks. It is caused by IBDV, a double-stranded RNA virus carrying its main antigenic epitopes on the capsid protein VP2. Our objective was to evaluate the immune response elicited by two heterologous prime-boost schemes combining an rMVA carrying the VP2 mature gene (rVP2) and a recombinant VP2 protein produced in Nicotiana benthamiana (pVP2), and to compare them with the performance of the homologous pVP2-pVP2 scheme usually used in our laboratory. The SPF chickens immunized with the three evaluated schemes elicited significantly higher anti-VP2 antibody titers (p 0.001) and seroneutralizing titers (p 0.05) and had less T-cell infiltration (p 0.001), histological damage (p 0.001) and IBDV particles (p 0.001) in their bursae of Fabricius when compared with control groups. No significant differences were found between both heterologous schemes and the homologous one. However, the rVP2-pVP2 scheme showed significantly higher anti-VP2 antibody titers than pVP2-rVP2 and a similar tendency was found in the seroneutralization assay. Conversely, pVP2-rVP2 had the best performance when evaluated through bursal parameters despite having a less potent humoral immune response. These findings suggest that the order in which rVP2 and pVP2 are combined can influence the immune response obtained. Besides, the lack of a strong humoral immune response did not lessen the ability to protect from IBDV challenge. Therefore, further research is needed to evaluate the mechanisms by which these immunogens are working in order to define the combination that performs better against IBDV. (C) 2016 Elsevier Ltd. All rights reserved.
机译：在过去几年中，已经评估了针对传染性法氏囊病病毒 （IBDV） 的不同免疫原，例如亚单位、DNA 或活病毒载体疫苗。然而，使用重组改良牛痘安卡拉病毒 （rMVA） 的异源初免-加强方法在哺乳动物和鸡中都显示出有希望的结果，但尚未尝试过针对这种病原体的试验。IBD是一种高度传染性和免疫抑制性的家禽疾病，主要影响雏鸡。它是由IBDV引起的，IBDV是一种双链RNA病毒，其主要抗原表位位于衣壳蛋白VP2上。我们的目的是评估两种异源初免-加强方案引起的免疫反应，这些方案结合了携带VP2成熟基因（rVP2）的rMVA和本氏烟草（pVP2）中产生的重组VP2蛋白，并将它们与我们实验室中通常使用的同源pVP2-pVP2方案的性能进行比较。与对照组相比，采用3种评估方案免疫的SPF鸡在法布里修斯滑囊中显著提高了抗VP2抗体滴度（p < 0.001）和血清中和滴度（p < 0.05），T细胞浸润（p < 0.001）、组织学损伤（p < 0.001）和IBDV颗粒（p < 0.001）。异源方案和同源方案之间没有发现显著差异。然而，rVP2-pVP2方案显示出显著高于pVP2-rVP2的抗VP2抗体滴度，并且在血清中和试验中发现了类似的趋势。相反，尽管 pVP2-rVP2 的体液免疫反应较弱，但在通过滑囊参数进行评估时具有最佳性能。这些发现表明，rVP2 和 pVP2 结合的顺序可以影响获得的免疫反应。此外，缺乏强烈的体液免疫反应并没有降低抵御IBDV攻击的能力。因此，需要进一步的研究来评估这些免疫原起作用的机制，以确定对IBDV表现更好的组合。（C） 2016 爱思唯尔有限公司保留所有权利。

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