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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >Rapid assays of clozapine and its metabolites in dried blood spots by liquid chromatography and microextraction by packed sorbent procedure
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Rapid assays of clozapine and its metabolites in dried blood spots by liquid chromatography and microextraction by packed sorbent procedure

机译：通过液相色谱法和填充吸附剂程序微萃取快速测定干燥血斑中的氯氮平及其代谢物

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摘要

A novel analytical approach has been developed for the determination of clozapine and its metabolites in dried blood spots on filter paper, using a chromatographic method coupled with a microextraction by packed sorbent procedure. The analytes were separated on a RP-C18 column using a mobile phase composed of 20 methanol, 16 acetonitrile and 64 aqueous phosphate buffer. Coulometric detection was used, setting the guard cell at +0.050V, the first analytical cell at -0.200V and the second analytical cell at +0.500V. Clozapine and its metabolites were extracted from dried blood spots with phosphate buffer and, then, a microextraction by packed sorbent procedure for the sample clean-up was implemented obtaining good extraction yields. The calibration curve was linear over the 2.5~(-1)000ngmL~(-1) blood concentration ranges for all the analytes. The method validation gave satisfactory results in terms of sensitivity, precision, selectivity and accuracy. The analytical method was successfully applied to dried blood spots from several psychiatric patients for therapeutic drug monitoring purpose.
机译：已经开发了一种新的分析方法，用于测定滤纸上干燥血斑中的氯氮平及其代谢物，使用色谱法和填充吸附剂程序的微萃取。使用由20%甲醇、16%乙腈和64%磷酸盐水溶液组成的流动相在RP-C18色谱柱上分离分析物。使用库鲁法检测，将保护池设置为+0.050V，第一个分析池设置为-0.200V，第二个分析池设置为+0.500V。用磷酸盐缓冲液从干燥的血斑中提取氯氮平及其代谢物，然后通过填充吸附剂进行样品净化程序进行微萃取，获得良好的提取收率。所有分析物的校准曲线在2.5~（-1）000ngmL~（-1）血药浓度范围内呈线性。方法验证在灵敏度、精密度、选择性和准确度方面均取得了令人满意的结果。该分析方法成功应用于数名精神病患者的干血斑，用于药物治疗监测。

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