Microfluidics for cryopreservation

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Microfluidics for cryopreservation

机译：用于冷冻保存的微流体技术

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Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell -specific protocols for the addition/removal of cryoprotective agents and freez-thaw cycles. Microfluidic platforms can revolutionize cryopreservation by providing new tools to manipulate and screen cells at micro/nano scales, which are presently difficult or impossible with conventional bulk approaches. This review describes applications of microfluidic tools in cell manipulation, cryoprotective agent exposure, programmed freezing/thawing, vitrification, and in situ assessment in cryopreservation, and discusses achievements and challenges, providing perspectives for future development. (C) 2017 Elsevier Inc. All rights reserved.

机译：冷冻保存在临床和科学研究中具有实用性，但实施非常复杂，包括用于添加/去除冷冻保护剂和冻融循环的劳动密集型细胞特异性方案。微流控平台可以通过提供在微/纳米尺度上操作和筛选细胞的新工具来彻底改变冷冻保存，而这在传统的批量方法中目前是困难或不可能的。本文综述了微流控工具在细胞操作、冷冻保护剂暴露、程序冻融、玻璃化和原位评估等冷冻保存中的应用，并讨论了成果和挑战，为未来的发展提供了展望。（C） 2017 爱思唯尔公司保留所有权利。

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《Biotechnology Advances: An International Review Journal》 |2017年第2期|323-336|共14页
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正文语种英语
中图分类生物工程学（生物技术）;
关键词
Microfluidics; Cryopreservation; Freezing/thawing; Oyoprotective agent loading/unloading; Vitrification;

机译：微流控;冷冻保存;冻融;Oyo保护剂装卸;玻璃化;

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1. Oocyte cryopreservation versus ovarian tissue cryopreservation for adult female oncofertility patients: a cost-effectiveness study [J] . Chung Esther H., Lim Stephanie L., Myers EvanMoss Haley A.Acharya Kelly S. Journal of assisted reproduction and genetics . 2021,第9期

机译：Oocyte cryopreservation versus ovarian tissue cryopreservation for adult female oncofertility patients: a cost-effectiveness study
2. Modified light spectral conditions prior to cryopreservation alter growth characteristics and cryopreservation success of potato (Solanum tuberosum L.) shoot tips in vitro [J] . Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants . 2017,第2期

机译：Modified light spectral conditions prior to cryopreservation alter growth characteristics and cryopreservation success of potato (Solanum tuberosum L.) shoot tips in vitro
3. The added value of embryo cryopreservation to cumulative ongoing pregnancy rates per IVF treatment: is cryopreservation worth the effort? [J] . de Jong D, Eijkemans MJ, Beckers NGPruijsten RVFauser BCMacklon NS Journal of assisted reproduction and genetics . 2002,第12期

机译：The added value of embryo cryopreservation to cumulative ongoing pregnancy rates per IVF treatment: is cryopreservation worth the effort?
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Microfluidics for cryopreservation

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