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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Temperature gradient focusing in a PDMS/glass hybrid microfluidic chip
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Temperature gradient focusing in a PDMS/glass hybrid microfluidic chip

机译:PDMS /玻璃混合微流体芯片中的温度梯度聚焦

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This paper reports the application of temperature gradient focusing (TGF) in a PDMS/glass hybrid microfluidic chip. With TGF, by the combination of a temperature gradient along a microchannel, an applied electric field, and a buffer with a temperature-dependent ionic strength, analytes are focused by balancing their electrophoretic velocities against the bulk velocity of the buffer containing the analytes. In this work, Oregon Green 488 carboxylic acid was concentrated approximately 30 times as high as the initial concentration in 45 s at moderate electric strength of 70 V/cm and a temperature gradient of 55 degrees C across the PDMS/glass hybrid microfluidic chip with a 1 cm long capillary.
机译:本文报道了温度梯度聚焦(TGF)在PDMS /玻璃混合微流控芯片中的应用。使用TGF,通过沿着微通道的温度梯度,施加的电场和具有随温度变化的离子强度的缓冲液的组合,可以通过平衡电泳速度与包含分析物的缓冲液的整体速度来平衡分析物。在这项工作中,在70伏/厘米的适度电强度和55摄氏度的温度梯度下,将Oregon Green 488羧酸在45 s内浓缩至初始浓度的30倍,初始浓度约为初始浓度的30倍。 1厘米长的毛细管。

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