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首页> 外文期刊>Clinical and diagnostic laboratory immunology >groEL encodes a highly antigenic protein in Burkholderia pseudomallei.
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groEL encodes a highly antigenic protein in Burkholderia pseudomallei.

机译：groEL 在 Burkholderia pseudomallei 中编码一种高度抗原的蛋白。

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摘要

No recombinant protein is available for serodiagnosis of melioidosis. In this study, we report the cloning of the groEL gene, which encodes an immunogenic protein of Burkholderia pseudomallei. Bidirectional DNA sequencing of groEL revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kDa. Basic Local Alignment Search Tool analysis showed that the putative protein encoded by groEL is homologous to the chaperonins encoded by the groEL genes of other bacteria. It has 98 amino acid identity with the GroEL of Burkholderia cepacia, 98 amino acid identity with the GroEL of Burkholderia vietnamiensis, and 82 amino acid identity with the GroEL of Bordetella pertussis. Furthermore, it was observed that patients with melioidosis develop a strong antibody response against GroEL, suggesting that the recombinant protein and its monoclonal antibody may be useful for serodiagnosis in patients with melioidosis and that the protein may represent a good cell surface target for host humoral immunity. Further studies in these directions would be warranted.
机译：没有重组蛋白可用于类鼻疽的血清诊断。在这项研究中，我们报告了groEL基因的克隆，该基因编码伯克霍尔德氏菌的免疫原性蛋白。groEL的双向DNA测序显示，该基因包含一个编码546个氨基酸残基的开放阅读框，预测分子量为57.1 kDa。基本局部比对搜索工具分析表明，groEL编码的推定蛋白与其他细菌的groEL基因编码的伴侣蛋白同源。它与洋葱伯克霍尔德菌的 GroEL 具有 98% 的氨基酸同一性，与越南伯克霍尔德氏菌的 GroEL 具有 98% 的氨基酸同一性，与百日咳博德特氏菌的 GroEL 具有 82% 的氨基酸同一性。此外，观察到类鼻疽患者对GroEL产生强烈的抗体反应，这表明重组蛋白及其单克隆抗体可能有助于类鼻疽患者的血清学诊断，并且该蛋白可能代表宿主体液免疫的良好细胞表面靶标。有必要在这些方向上进行进一步的研究。

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