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首页> 外文期刊>molecular diagnosis >Cytogenetics, Immunostaining for Fibroblast Growth Factors, p53 Sequencing, and Clinical Features of Two Cases of Cystosarcoma Phyllodes
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Cytogenetics, Immunostaining for Fibroblast Growth Factors, p53 Sequencing, and Clinical Features of Two Cases of Cystosarcoma Phyllodes

机译：细胞遗传学、成纤维细胞生长因子免疫染色、p53测序及2例叶状囊肉瘤的临床特征

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Background:We present cytogenetics and fibroblast growth factor immunohisto-chemistry in one case of cystosarcoma phyllodes with localized disease and one with metastatic spread. Thep53gene was sequenced in the malignant case.Materials and Results:Karyotype analysis used trypsin-Giemsa banding. Immunohistochemistry of FGF1, FGF2, FGFR1 and p53 used avidin-biotin detection of the primary antibody. One case had a mosaic female karyotype and three clones: one normal, one with trisomy 7, and one with both trisomy 5 and a rearranged chromosome 1. In the second case, a resected pulmonary metastasis had the karyotype 43-47,XX,plus;mar1,plus;mar2lsqb;6rsqb;/43-46,XX,plus;del(7)(p10)lsqb;3rsqb;,plus;mar2lsqb;1rsqb;lsqb;cp3rsqb;/46,XXlsqb;10rsqb;. These tumors expressed FGF1, FGF2, and FGFR1. The malignant case showed immunostaining for p53 protein, but a wild-type gene sequence.Conclusion:The karyotype of cystosarcoma phyllodes is complex, with wide case-to-case variation. These tumors express members of the FGF family. Metastatic behavior can occur in the presence of a wild-typep53gene.
机译：背景： 我们在一例具有局限性疾病的囊肉瘤叶状瘤和一例具有转移性扩散的囊肉瘤叶状瘤中介绍了细胞遗传学和成纤维细胞生长因子免疫组织化学。在恶性病例中对 thep53gene 进行测序。材料和结果： 使用胰蛋白酶-Giemsa 条带进行核型分析。FGF1、FGF2、FGFR1 和 p53 的免疫组化使用亲和素-生物素检测一抗。1例具有嵌合雌性核型和3个克隆：1个正常，1个为7三体，1个为5三体和1号染色体重排。在第二例中，切除的肺转移瘤的核型为 43-47，XX，+mar1，+mar2[6]/43-46，XX，+del（7）（p10）[3]，+mar2[1][cp3]/46，XX[10]。这些肿瘤表达 FGF1、FGF2 和 FGFR1。恶性病例显示 p53 蛋白免疫染色，但显示野生型基因序列。结论：囊肉瘤叶状体的核型复杂，因病例间差异大。这些肿瘤表达FGF家族的成员。在存在野生型p53基因的情况下，可发生转移行为。

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