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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Enzymatic in-capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection.
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Enzymatic in-capillary derivatization for glucose determination by electrophoresis with spectrophotometric detection.

机译：酶促毛细管内衍生化，用于通过电泳和分光光度检测法测定葡萄糖。

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摘要

The following paper compares several procedures of in-capillary bienzymatic derivatization with regard to glucose determination with the use of glucose oxidase and horseradish peroxidase. The procedures discussed below include continous contact in the capillary, plug-plug injection, and sequential injection with incubation in the capillary inlet. The reaction of hydrogen peroxide catalyzed by peroxidase was performed using two different substrates. The best results were achieved for nicotinamide adenine dinucleotide, reduced disodium salt (NADH) acting both as a chromogenic reagent and a substrate for peroxidase, while the method employed was sequential injection and incubation at the capillary inlet. The LOD was estimated to be 25 nM with a linear response up to 0.1 muM.
机译：以下论文比较了使用葡萄糖氧化酶和辣根过氧化物酶进行葡萄糖测定时毛细管内双酶衍生化的几种方法。下面讨论的程序包括毛细管中的连续接触，塞-塞注入和在毛细管入口中孵育的顺序注入。使用两种不同的底物进行过氧化物酶催化的过氧化氢反应。烟酰胺腺嘌呤二核苷酸，还原二钠盐（NADH）既用作生色试剂又是过氧化物酶的底物，则获得了最佳结果，而所采用的方法是在毛细管入口处依次注射和孵育。 LOD估计为25 nM，线性响应最高达0.1μM。

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