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首页> 外文期刊>Cell and Tissue Research >Isolation, characterization and immunomodulatory-associated gene transcription of Wharton's jelly-derived multipotent mesenchymal stromal cells at different trimesters of cow pregnancy
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Isolation, characterization and immunomodulatory-associated gene transcription of Wharton's jelly-derived multipotent mesenchymal stromal cells at different trimesters of cow pregnancy

机译：奶牛妊娠不同孕期沃顿氏果冻来源的多能间充质基质细胞的分离、表征和免疫调节相关基因转录

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摘要

The possibility of isolating bovine mesenchymal multipotent stromal cells (MSCs) from fetal adnexa is an interesting prospect due to the potential use of these cells in biotechnological applications. However, little is known about the properties of these progenitor cells in bovine species. Wharton's jelly (WJ) MSC cells were obtained from the umbilical cord of bovine fetuses at three different stages of pregnancy and divided into groups 1, 2 and 3 according to gestational trimester. Cell morphology, from the three stages of pregnancy, typically appeared fibroblast-like spindle-shaped, presenting the same viability and number. Moreover, the proliferative ability of T-cells in response to a mitogenic stimulus was suppressed when WJMSC cells were added to the culture. Multilineage properties were confirmed by their ability to undergo adipogenic, osteogenic/chondrogenic and neurogenic differentiation. Mesenchymal phenotyping, CD105+, CD29+, CD73+ and CD90+ cell markers were detected in all three cell groups, yet these markers were considered more expressed in MSCs of group 2 (p < 0.005). Expression of cytokines IL2, IL6RR, INFAC, INFB1, IFNG, TNF and LTBR were downregulated, whereas IL1F10 expression was upregulated in all tested WJMSCs. The present study demonstrated that WJMSCs harvested from the bovine umbilical cord at different gestational stages showed proliferative capacity, immune privilege and stemness potential.
机译：由于这些细胞在生物技术应用中的潜在用途，从胎儿附件中分离牛间充质多能基质细胞 （MSC） 的可能性是一个有趣的前景。然而，人们对这些祖细胞在牛物种中的特性知之甚少。沃顿氏果冻（WJ）MSC细胞取自妊娠3个不同阶段的牛胎儿脐带，根据妊娠期分为第1、2、3组。从怀孕的三个阶段来看，细胞形态通常呈成纤维细胞样纺锤形，呈现相同的活力和数量。此外，当将WJMSC细胞添加到培养物中时，T细胞响应有丝分裂刺激的增殖能力受到抑制。多谱系特性通过它们经历成脂、成骨/软骨和神经源性分化的能力得到证实。在所有三个细胞组中都检测到间充质表型、CD105+、CD29+、CD73+ 和 CD90+ 细胞标志物，但这些标志物被认为在第 2 组的 MSC 中表达更多 （p < 0.005）。细胞因子IL2、IL6RR、INFAC、INFB1、IFNG、TNF和LTBR的表达均下调，而IL1F10表达在所有测试的WJMSCs中均上调。本研究表明，不同妊娠期从牛脐带采集的WJMSCs具有增殖能力、免疫特权和干性潜力。

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