...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electroanalysis >Metrological Aspects of Glucose Measurements by Biosensors
【24h】

Metrological Aspects of Glucose Measurements by Biosensors

机译：生物传感器进行葡萄糖测量的计量方面

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In a chronoamperometric measurement the chemical activity of glucose is directly determined using a glucose sensitive biosensor based on glucose oxidase and an efficient mediator. Within the physiological range of 2 mmol kg(-1) and 10 mmol kg(-1) glucose in aqueous solutions behaves almost ideally and the chemical activity equals the glucose molality, the amount of glucose per unit mass of water, within the estimated measurement uncertainty. In physiological samples the equality between chemical activity and glucose molality is destroyed and has to be corrected for. Gravimetrically prepared mixtures of high purity glucose in buffered aqueous solutions are used as standards for calibration of the chronoamperometric measurement setup. Based on the model equation for the measurand aqueous or physiological sample, all significant sources of uncertainty are identified, their magnitude estimated from published and experimental data and finally combined to give the uncertainty in the reported value of the glucose molality. It is found, that the combined uncertainty of the glucose molality comprises mainly uncertainty contributions from the nonideal behavior, the chronoamperometric measurement setup, from the purity of glucose used and from the chemical composition of the test sample. The expanded uncertainty is below 2% rel., the glucose content determined by the bioelectrochemical measurements thus competes well with today's considered most accurate reference method Isotope Dilution Mass Spectrometry. Advantageous of the presented electroanalytical method is the direct measure of the glucose molality without prior sample preparation and dilution.
机译：在计时电流测量中，使用基于葡萄糖氧化酶和有效介体的葡萄糖敏感生物传感器直接测定葡萄糖的化学活性。在2 mmol kg（-1）和10 mmol kg（-1）的生理范围内，水溶液中的葡萄糖表现理想，化学活性等于葡萄糖摩尔浓度，即单位质量水的葡萄糖量（在估计的测量范围内）不确定。在生理样品中，化学活性和葡萄糖摩尔浓度之间的相等性被破坏，必须对其进行校正。重量分析法制备的高纯度葡萄糖在缓冲水溶液中的混合物用作校准计时电流法测量设置的标准。根据被测水样或生理样品的模型方程式，可以确定所有重要的不确定性来源，并根据发表的数据和实验数据对它们的幅度进行估算，最后结合起来得出报告的葡萄糖摩尔浓度的不确定性。已发现，葡萄糖摩尔浓度的综合不确定度主要包括非理想行为，计时电流法测量设置，所用葡萄糖的纯度以及测试样品的化学成分所产生的不确定度。扩大的不确定度低于2％rel。，因此，通过生物电化学测量确定的葡萄糖含量与当今被认为是最准确的参考方法同位素稀释质谱法相竞争。所提出的电分析方法的优点是无需事先进行样品制备和稀释即可直接测量葡萄糖的摩尔浓度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号