Mutant MyoD Lacking Cdc2 Phosphorylation Sites Delays M-Phase Entry

Lionel A. J. Tintignac; Valentina Sirri; Marie Pierre LeibovitchYann LecluseMaria CastedoDidier MetivierGuido KroemerSerge A/ :eobpvotcj

首页> 外文期刊>Molecular and Cellular Biology >Mutant MyoD Lacking Cdc2 Phosphorylation Sites Delays M-Phase Entry

【24h】

Mutant MyoD Lacking Cdc2 Phosphorylation Sites Delays M-Phase Entry

机译：

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

The transcription factors MyoD and Myf-5 control myoblast identity and differentiation. MyoD and Myf-5 manifest opposite cell cycle-specific expression patterns. Here, we provide evidence that MyoD plays a pivotal role at the G2/M transition by controlling the expression of p21Waf1/Cip1 (p21), which is believed to regulate cyclin B-Cdc2 kinase activity in G2. In growing myoblasts, MyoD reaccumulates during G2 concomitantly with p21 before entry into mitosis; MyoD is phosphorylated on Ser5 and Ser200 by cyclin B-Cdc2, resulting in a decrease of its stability and down-regulation of both MyoD and p21. Inducible expression of a nonphosphorylable MyoD A5/A200 enhances the MyoD interaction with the coactivator P/CAF, thereby stimulating the transcriptional activation of a luciferase reporter gene placed under the control of the p21 promoter. MyoD A5/A200 causes sustained p21 expression, which inhibits cyclin B-Cdc2 kinase activity in G_2 and delays M-phase entry. This G_2 arrest is not observed in p21~(-/-) cells. These results show that in cycling cells MyoD functions as a transcriptional activator of p21 and that MyoD phosphorylation is required for G_2/M transition.

著录项

来源
《Molecular and Cellular Biology》 |2004年第4期|1809-1821|共13页
作者
Lionel A. J. Tintignac; Valentina Sirri; Marie Pierre LeibovitchYann LecluseMaria CastedoDidier MetivierGuido KroemerSerge A/ :eobpvotcj;
展开▼
作者单位

Laboratoire de Genetique Oncologique, Institut Gustave Roussy, 94805 Villejuif, France;

Service commun de cytofluorometrie, Institut Gustave Roussy, 94805 Villejuif, France;

Laboratoire de Genetique Oncologique, Institut Gustave Roussy, 94805 Villejuif, France, Institut Gustave Roussy, 94805 Villejuif, France;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类分子生物学;
关键词

Mutant MyoD Lacking Cdc2 Phosphorylation Sites Delays M-Phase Entry

摘要

著录项

相关主题

期刊订阅