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首页> 外文期刊>European journal of gastroenterology and hepatology >Increased production of vascular endothelial growth factor by peripheral blood mononuclear cells in patients with inflammatory bowel disease.
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Increased production of vascular endothelial growth factor by peripheral blood mononuclear cells in patients with inflammatory bowel disease.

机译：炎性肠病患者外周血单个核细胞增加血管内皮生长因子的产生。

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BACKGROUND: Vascular endothelial growth factor (VEGF) is a potent angiogenic, vascular permeability-enhancing cytokine with overexpression in various pathological disorders, including tumour growth, chronic inflammation and tissue repair. Recent studies have shown significantly increased serum levels of VEGF in patients with inflammatory bowel disease. The origin of the circulating VEGF is still unknown. The present investigation examines the VEGF production by peripheral blood mononuclear cells (PBMCs) in patients with inflammatory bowel disease. METHODS: VEGF levels were measured in culture supernatants of unstimulated PBMCs of 27 patients with inflammatory bowel disease and 10 healthy volunteers using a solid phase ELISA. In addition, VEGF serum levels were determined. RESULTS: PBMCs of both active Crohn's disease patients (1142.6+/-483.9 pg/ml, P < 0.001, n = 12) and active ulcerative colitis patients (748.0+/-637.6 pg/ml, P = 0.006, n = 4) produced significantly higher amounts of VEGF compared with PBMCs of healthy volunteers (113.4+/-101.8 pg/ml, n = 10). In addition, there was a significantly increased VEGF production by PBMCs of patients with active disease compared with PBMCs of patients with quiescent Crohn's disease (261.6+/-254.8 pg/ml, P < 0.001, n = 7) and inactive ulcerative colitis (147.7+/-100.3 pg/ml, P = 0.02, n = 4). There was no significant difference in VEGF release between patients with inactive inflammatory bowel disease and healthy controls. CONCLUSIONS: Significantly increased VEGF production by PBMCs was found in patients with active Crohn's disease and active ulcerative colitis. The study helps to clarify one of the origins of the significantly enhanced VEGF serum levels in patients with active inflammatory bowel disease observed in recent studies.
机译：背景：血管内皮生长因子（VEGF）是一种有效的血管生成，增强血管通透性的细胞因子，在多种病理疾病（包括肿瘤生长，慢性炎症和组织修复）中都有过表达。最近的研究表明，炎症性肠病患者的血清VEGF水平显着增加。循环中VEGF的起源仍是未知的。本研究检查了炎症性肠病患者外周血单个核细胞（PBMC）产生的VEGF。方法：采用固相ELISA法检测27例炎症性肠病患者和10例健康志愿者的未刺激PBMCs培养上清中的VEGF水平。另外，测定VEGF血清水平。结果：活动性克罗恩病患者（1142.6 +/- 483.9 pg / ml，P <0.001，n = 12）和活动性溃疡性结肠炎患者（748.0 +/- 637.6 pg / ml，P = 0.006，n = 4）的PBMC与健康志愿者的PBMC相比，VEGF产生的VEGF量明显更高（113.4 +/- 101.8 pg / ml，n = 10）。此外，与患有静止性克罗恩病（261.6 +/- 254.8 pg / ml，P <0.001，n = 7）和非活动性溃疡性结肠炎（147.7）的患者的PBMC相比，患有活动性疾病的患者的PBMC显着增加了VEGF的产生+/- 100.3 pg / ml，P = 0.02，n = 4）。非活动性炎症性肠病患者与健康对照组之间的VEGF释放无显着差异。结论：活动性克罗恩病和活动性溃疡性结肠炎患者发现PBMCs可显着增加VEGF的产生。这项研究有助于弄清最近研究中发现的活动性炎症性肠病患者VEGF血清水平显着升高的原因之一。

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