Development of 3-hydroxybutyrate dehydrogenase enzyme biosensor based on carbon nanotube-modified screen-printed electrode

Fahimeh Khorsand; Siavash Riahi; Solmaz Eynollahi FardSoheila KashanianAli NaeemyBagher LarijaniKobra Omidfar

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Development of 3-hydroxybutyrate dehydrogenase enzyme biosensor based on carbon nanotube-modified screen-printed electrode

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Precise detection of 3-hydroxybutyrate (HB) in biological samples is of great importance for management of diabetic patients. In this study, an HB biosensor based on single-walled carbon nanotubes (SWCNTs)-modified screen-printed electrode (SPE) was developed to determine the concentration of HB in serum. The specific detecting enzyme, HB dehydrogenase, was physically immobilised on SWCNTs deposited on the surface of SPEs. The electrochemical measurement of HB that involved cyclic voltammetry was based on the signal produced by β-nicotinamide adenine dinucleotide (NADH), one of the products of the enzymatic reaction. The application of SWCNT reduced the oxidation potential of NADH to about -0.05 V. Electrochemical measurements showed that the response of this biosensor had relevant good linearity in the range of 0.1-2 mM with a low detection limit of 0.009 mM. Investigation of biosensor response in the presence of interfering molecules verified its specificity. Furthermore, the study of long-term stability demonstrated the acceptable efficiency of this biosensor for about 100 days.

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《IET nanobiotechnology》 |2013年第1期|1-6|共6页
作者
Fahimeh Khorsand; Siavash Riahi; Solmaz Eynollahi FardSoheila KashanianAli NaeemyBagher LarijaniKobra Omidfar;
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作者单位

Endocrine and Metabolism Research Center, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, I.R. Iran;

Institute of Petroleum Engineering, College of Engineering, University of Tehran, Tehran, I.R. Iran;

Department of Chemistry, Sensor and Biosensor Research Center (SBRC) and Nanoscience and Nanotechnology Research Center (NNRC), Faculty of Science, Razi University, Kermanshah, I.R. Iran;

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正文语种英语
中图分类电工技术;
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Development of 3-hydroxybutyrate dehydrogenase enzyme biosensor based on carbon nanotube-modified screen-printed electrode

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