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Genetic variation and an estimation of effective population size in the pearl oyster Pinctada martensii

机译:珍珠牡蛎Pinctada martensii的遗传变异和有效种群的估计

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In September 2011, two wild populations of pearl oysters were collected separately from Daya Bay, Shenzhen, and from Beibu Bay, Beihai, China. In April 2012, three cultured stocks were established by sampling breeders from the third-generation selected lines. The genetic variation within the five stocks was analyzed using eight SSR primer pairs. The effective population sizes of the five stocks were analyzed using the sibship reconstruction and multiple summary statistics methods. A total of 22-29 alleles were observed in the five stocks, with two to five alleles per locus and means ranging from 2.750 to 3.625 alleles per locus. The average expected and observed heterozygosities ranged from 0.3387 to 0.5029 and from 0.2917 to 0.4219, respectively. The effective population sizes of the five stocks ranged from 11.0 to 20.0 when estimated using sibship reconstruction and from 13.7 to 179.4 when estimated using multiple summary statistics. The effective population sizes estimated in the three cultured stocks were smaller than the number of breeders used to produce these stocks. Thus, it may be necessary to augment the number of breeders used to produce progeny stocks in selective breeding programs
机译:2011年9月,分别从深圳大亚湾和中国北海北部湾采集了两个野生牡蛎种群。 2012年4月,通过对第三代选定品系的育种者进行采样,建立了三个养殖种群。使用八对SSR引物对五种种群中的遗传变异进行了分析。使用同胞重建和多重汇总统计方法分析了五只种群的有效种群数量。在五种种群中共观察到22-29个等位基因,每个基因座2至5个等位基因,平均每个基因座范围为2.750至3.625个等位基因。平均预期和观察到的杂合度分别为0.3387至0.5029和0.2917至0.4219。使用同胞重建来估算时,五只种群的有效种群数量在11.0到20.0之间,而在使用多个汇总统计数据估算时,在有效种群中从13.7到179.4之间。在三种养殖种群中估计的有效种群数量小于用于生产这些种群的育种者数量。因此,可能有必要在选择性育种计划中增加用于生产后代种群的育种者数量

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