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首页> 外文期刊>Genetic Resources and Crop Evolution >Analysis of genetic diversity in the tuber mustard (Brassica juncea var. tumida Tsen et Lee) in the Yangtze river basin of China
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Analysis of genetic diversity in the tuber mustard (Brassica juncea var. tumida Tsen et Lee) in the Yangtze river basin of China

机译：长江流域芥菜（Brassica juncea var。tumida Tsen et Lee）的遗传多样性分析

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In the present study, analyses of SSR molecular markers were performed to investigate the genetic diversity of 133 tuber mustard cultivars. Eighty-one pairs of SSR primers from a total of 600 in Brassica produced stable amplified bands. In addition, 810 bands were detected among the cultivars, and 724 of those were polymorphic (89.38 %). The average number of bands per locus was 10.0 with a range from 5 to 16. Shannon's information index for each SSR locus varied from 0.52 to 3.72, with an average of 2.74. The coefficients of genetic similarity in the SSR marker patterns among the 133 cultivars ranged from 0.77 to 0.91, with an average of 0.85. The cluster analysis showed that the cultivars could be classified into six clusters when the genetic similarity was 0.83, with 90.23 % of the cultivars included in Clusters 5 and 6. Principal component analysis was carried based on the SSR data. The results showed that the first three principal components could explain the genetic variation with 85.47, 0.67, and 0.61 %, and the 133 cultivars could be divided into six clusters according to the nearest phylogenetic relationship. It was indicated that the similarity was high and the genetic diversity was narrow among the 133 mustard tuber cultivars. 360 individuals from 24 cultivars were analyzed to reveal the genetic structure and genetic diversity within cultivars. A total of 925 alleles were detected in the 24 cultivars. Estimates of the mean number of alleles 'A', the effective allelic number 'A(e)', the observed heterozygosity 'H-o', and expected heterozygosity 'H-e' were 6.0, 3.6, 0.64, and 0.37, respectively. An obvious genetic deviation from Hardy-Weinberg expectation was observed both among and within cultivars and a considerable genetic variation was revealed within rather than among cultivars. It is necessary to broaden the genetic basis of the breeding germplasm in tuber mustard. Based on their geographical distributions, the tuber mustard cultivars in this study can be divided into up-Yangtze river, mid-Yangtze river, and down-Yangtze river groups. Genetic diversity was highest in mid-Yangtze river group, followed by up-Yangtze river group, and then down-Yangtze river group. It was presumed that the origin center or genetic diversity center of tuber mustard was mid-Yangtze river, and the crop was transmitted along the Yangtze river in both directions.
机译：在本研究中，进行了SSR分子标记分析，以研究133个块茎芥菜品种的遗传多样性。来自芸苔属的总共600对中的81对SSR引物产生稳定的扩增条带。此外，在品种中检测到810个条带，其中724个是多态性的（89.38％）。每个基因座的平均条带数为10.0，范围为5到16。每个SSR基因座的Shannon信息指数从0.52到3.72不等，平均值为2.74。 133个品种中SSR标记模式的遗传相似系数在0.77至0.91之间，平均为0.85。聚类分析表明，当遗传相似度为0.83时，该品种可分为6个品种，其中5个和6个品种占90.23％。基于SSR数据进行主成分分析。结果表明，前三个主要成分可以解释遗传变异，分别为85.47、0.67和0.61％，根据最近的系统发生关系，可以将133个品种分为六个簇。结果表明，在133个芥菜块茎品种中，其相似度很高，遗传多样性较窄。分析了来自24个品种的360个个体，以揭示其内部的遗传结构和遗传多样性。在24个品种中共检测到925个等位基因。等位基因“ A”的平均数，有效等位基因数“ A（e）”，观察到的杂合度“ H-o”和预期杂合度“ H-e”的估计分别为6.0、3.6、0.64和0.37。在品种内部和内部都观察到明显的与Hardy-Weinberg期望的遗传偏差，并且在品种内部而不是品种之间发现了相当大的遗传变异。有必要拓宽块茎芥菜种质的遗传基础。根据其地理分布，本研究中的块茎芥菜品种可分为长江上游，长江中游和下游长江三类。遗传多样性在长江中游群最高，其次是长江上游群，然后是下游长江群。据推测，块茎芥菜的起源中心或遗传多样性中心在长江中游，作物沿长江双向传播。

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