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Effects of cryopreservation on germinability of olive (Olea europaea L.) pollen

机译：冷冻保存对橄榄（Olea europaea L.）花粉萌发的影响

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摘要

Long term storage of viable pollen is important for bank germplasm constitution to preserve resources that can be used in breeding programs, biotechnologies and genetic engineering. Pollen from 12 olive (Olea europaea L.) cultivars was stored for 1 year in liquid nitrogen at -196A degrees C. The morphology of pollen grains and germination rates on fresh and long term stored pollen were observed. Results on in vitro pollen germinability, both before and after cryopreservation, showed highly significant responses among the 12 cultivars. The relationship between germinability and pollen grain size did not reveal any significant relationships in both treatments. Our findings will contribute to the improvement of olive pollen preservation and lead to find a more efficient method for its long term storage, while retaining its germinability.
机译：长期储存有活力的花粉对于银行种质的构成至关重要，以保存可用于育种计划，生物技术和基因工程的资源。将来自12个橄榄（Olea europaea L.）品种的花粉在-196A摄氏度的液氮中保存1年。观察到新鲜和长期保存的花粉的花粉粒形态和发芽率。冷冻保存之前和之后的体外花粉可发芽性结果在12个品种中显示出高度显着的响应。在两种处理中，可发芽性与花粉粒度之间的关系均未显示任何显着关系。我们的发现将有助于改善橄榄花粉的保存性，并导致寻找一种更有效的方法来长期保存橄榄花粉，同时保持其可发芽性。

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