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首页> 外文期刊>Genetic Resources and Crop Evolution >Divergent evolution of wild and cultivated subspecies of Triticum timopheevii as revealed by the study of PolA1 gene
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Divergent evolution of wild and cultivated subspecies of Triticum timopheevii as revealed by the study of PolA1 gene

机译：PolA1基因的研究揭示了野生小麦和栽培小麦的亚种的不同进化

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Triticum timopheevii (genome symbol AAGG) comprises two subspecies, cultivated ssp. timopheevii, and wild ssp. armeniacum. These two subspecies are considered as allotetraploids of AA genome from Triticum diploid species and SS genome from Aegilops species. The difference in genome symbol (G vs. S) is due to wide genetic variations among four SS genome species, Ae. bicornis, Ae. longissima, Ae. searsii, and Ae. speltoides. In order to study the origin of T. timopheevii, we compared 19th intron (PI19) sequence of the PolA1 gene, encoding the largest subunit of RNA polymerase I. Two different sized DNA fragments containing PI19 sequences (PI19A and PI19G) were amplified both in ssp. timopheevii and ssp. armeniacum. Shorter PI19A (112 bp) sequences of both subspecies were identical to PI19 sequences of two AA species, T. monococcum and T. urartu. Interestingly, the longer PI19G (241-243 bp) sequences of ssp. armeniacum showed more similarity to PI19 sequences of Ae. speltoides whereas ssp. timopheevii showed more similarity to PI19 sequences of other three SS genome species. The results indicated that two subspecies of T. timopheevii, ssp. armeniacum or ssp. timopheevii, might have arisen independently by allotetraploidization of AA genome with Ae. speltoides or one of the remaining three Aegilops species, respectively.
机译：Triticum timopheevii（基因组符号AAGG）包括两个亚种，即栽培种。 timopheevii和野生ssp。亚美尼亚。这两个亚种被认为是Triticum二倍体物种的AA基因组和Aegilops物种的SS基因组的四倍体。基因组符号（G vs. S）的差异是由于四个SS基因组物种Ae之间的广泛遗传变异所致。 A.bicornis。 A. Longissima。西尔斯伊和爱speltoides。为了研究T.phephephei的起源，我们比较了PolA1基因的第19个内含子（PI19）序列，该序列编码RNA聚合酶I的最大亚基。两个包含PI19序列的不同大小的DNA片段（PI19A和PI19G）在ssp。提莫菲维和亚美尼亚。两个亚种的较短的PI19A（112 bp）序列与两个AA种（T. monococcum和T. urartu）的PI19序列相同。有趣的是，ssp的更长的PI19G（241-243 bp）序列。亚美尼亚与Ae的PI19序列显示出更多相似性。 speltoides而ssp。替莫非韦显示出与其他三个SS基因组物种的PI19序列更相似。结果表明，T。timopheevii，ssp。的两个亚种。亚美尼亚菊或ssp。替莫非韦可能是通过Ae基因的Ae基因异源四倍体化而独立产生的。 speltoides或剩下的三种埃及盾形动物之一。

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