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首页> 外文期刊>Genetic Resources and Crop Evolution >Development of EST-SSR in foxtail millet (Setaria italica).
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Development of EST-SSR in foxtail millet (Setaria italica).

机译:谷子(Setaria italica)EST-SSR的开发。

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摘要

The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic and transferable SSRs were also identified.
机译:本文报道了谷子(Setaria italica)中EST-SSR的开发,用于多态性和转移性研究。从1213个EST序列中,获得了30个SSR,并设计了用于26个SSR的引物。其中,四对SSR引物扩增了12个谷子小米品种中的一个多态性产物,以及一个谷子野生近缘种Setaria viridis,其中四个位点检测到10个等位基因,每个基因座检测到2.5个等位基因。此外,可以将十个SSR标记转移到其他九个禾本科物种。还确定了11个包含多态SSR和可转移SSR的EST的推定功能。

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