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Identification of molecular markers linked to the genes for purple grain color in wheat (Triticum aestivum)

机译:鉴定与小麦(Triticum aestivum)紫色粒色基因相关的分子标记

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摘要

Pericarp color in wheat is an important trait related to nutrients and health benefits. Identification of molecular markers closely linked to the grain color genes will help utilize pericarp trait in wheat breeding. Genetic studies involving F, F, and F populations from the cross Jizi 439 (purple grain)/Gao8901 (white grain) showed that the purple grain color was controlled by two genes. By using the bulked segregant analysis (BSA) approach, two DNA pools were established from the dark purple-grain and white-grain plants respectively. Among the 230 pairs of microsatellite primers used in this study, two markers showed polymorphism in DNA pools, parents, F and F populations. Linkage analysis indicated that the two markers Xgwm47 and Xgwm155 were linked with two purple pigment genes. One of the purple pigment genes is located on chromosome 2AS and is 34.7cM away from the marker Xgwm47, whereas another gene is mapped on 3AL with 14.7cM away from marker Xgwm155.
机译:小麦的果皮颜色是与营养和健康益处相关的重要特征。鉴定与谷物颜色基因紧密相关的分子标记将有助于在小麦育种中利用果皮特性。遗传的研究涉及来自十字Ji子439(紫色粒)/ Gao8901(白色粒)的F,F和F种群,紫色粒的颜色受两个基因控制。通过使用大量分离子分析法(BSA),分别从暗紫粒和白粒植物中建立了两个DNA库。在这项研究中使用的230对微卫星引物中,两个标记在DNA库中显示多态性,即亲本,F和F群体。连锁分析表明,两个标记Xgwm47和Xgwm155与两个紫色色素基因连锁。紫色色素基因之一位于2AS染色体上,距离标记Xgwm47 34.7cM,而另一个基因位于3AL上,距离标记Xgwm155 14.7cM。

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