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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic variation within and among populations of Orychophragmus violaceus (Cruciferae) in China as detected by ISSR analysis
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Genetic variation within and among populations of Orychophragmus violaceus (Cruciferae) in China as detected by ISSR analysis

机译：ISSR分析检测的中国紫堇（Cruciferae）种群内部和种群之间的遗传变异

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摘要

Orychophragmus violaceus, a ground covering plant that is widely distributed in China. It has both high economical value in food, forage, health care and ornamental value in gardening. In this study, the genetic diversity of 245 individuals from nine populations in China were investigated using the inter-simple sequence repeat markers. Of the 100 primers screened, eight were highly polymorphic. Using these primers, 162 discernible DNA fragments were generated with 150 (92.59%) being polymorphic, indicating a pronounced genetic variation at the species level. Also, there were high levels of polymorphism at the population level with the percentage of polymorphic bands ranging from 85.74 to 90.06%. Analysis of molecular variance showed that the genetic variation within populations was 80.80% and the variance among populations was 16.43%. The Nei's G (ST) (0.1643) and gene flow among populations (Nm = 2.5760) revealed large gene exchanges among populations. O. violaceus belongs to out-crossing plants. It is capable of reproducing by self-sowing, thus can influence population genetic structure. The pronounced genetic variation within populations tells us that O. violaceus is a proper plant for genetic research and that there is great potential of breeding this species for gardening.
机译：紫罗兰（Orychophragmus violaceus），一种在中国广泛分布的地被植物。它在食品，饲料，保健和园艺方面均具有很高的经济价值。在这项研究中，使用简单序列重复标记研究了来自中国九个种群的245个个体的遗传多样性。在筛选的100个引物中，有8个具有高度多态性。使用这些引物，产生了162个可识别的DNA片段，其中150个（92.59％）是多态性的，表明在物种水平上明显的遗传变异。而且，在人群水平上存在高水平的多态性，多态性条带的百分比在85.74至90.06％之间。分子变异分析表明，群体内的遗传变异为80.80％，群体间的变异为16.43％。 Nei's G（ST）（0.1643）和种群之间的基因流动（Nm = 2.5760）显示出种群之间的大量基因交换。 O. violaceus属于异交植物。它能够自我播种繁殖，因此可以影响种群的遗传结构。种群内明显的遗传变异告诉我们，紫罗兰色is草是适合进行遗传研究的植物，并且具有繁殖该种以供园艺的巨大潜力。

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