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首页> 外文期刊>Genetic Resources and Crop Evolution >Molecular cytogenetic characterization of the amphiploid between bread wheat and Psathyrostachys huashanica
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Molecular cytogenetic characterization of the amphiploid between bread wheat and Psathyrostachys huashanica

机译：面包小麦与华山番荔枝间二倍体的分子细胞遗传学表征

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A new wheat-Psathyrostachys huashanica amphiploid, PHW-SA, was characterized using molecular cytological tools to evaluate the potential utilization of P. huashanica for wheat improvement. PHW-SA pollen mother cells (PMCs) regularly revealed averagely 0.57 univalents, 24.51 ring bivalents, 3.19 rod bivalents and 0.01 trivalents per cell. Complete homologous chromosome pairing was seen in 81% of the PMCs, indicating a degree of cytological stability. P. huashanica C-banding karyotypes of PHW-SA displayed strong heterochromatin terminal bands at either or both ends. 24 P. huashanica chromosome arms showed telomeric bands. Using P. huashanica DNA as a probe and J-11 DNA as blocker, distinctive P. huashanica chromosomes, which were presented entirely greenish-yellow hybridization signals, were observed in a somatic metaphase of PHW-SA. GISH also revealed the chromosomal breakage and translocation occurring in the amphiploid PHW-SA, which may represent a centromeric fusion between P. huashanica and wheat chromosomes. Seeds storage proteins electrophoresis indicated that PHW-SA expressed some of P. huashanica specific gliadin and glutenin bands, and a few gliadin and glutenin bands of the parents disappeared and new bands appeared. The results indicated that the amphiploid PHW-SA could serve as novel germplasm sources for wheat improvement.
机译：利用分子细胞学工具表征了一种新的小麦-华沙参二倍体PHW-SA，以评估其对小麦改良的潜在利用。 PHW-SA花粉母细胞（PMC）通常每细胞平均显示0.57单价，24.51环二价，3.19杆二价和0.01三价。在81％的PMC中观察到完全同源的染色体配对，表明其细胞学稳定性。华山假单胞菌PHW-SA的C带核型在任一端或两端均显示较强的异染色质末端带。 huashanica的24个染色体臂显示端粒带。以华山疟原虫DNA为探针，以J-11 DNA为阻断剂，在PHW-SA的体细胞中期观察到独特的华山疟原虫染色体，呈完全黄绿色的杂交信号。 GISH还揭示了在二倍体PHW-SA中发生的染色体断裂和易位，这可能代表华山毕赤酵母和小麦染色体之间的着丝粒融合。种子贮藏蛋白电泳表明，PHW-SA表达了华山疫霉的一些麦醇溶蛋白和谷蛋白条带，亲本的麦醇溶蛋白和谷蛋白条带消失了，出现了新的条带。结果表明，二倍体PHW-SA可作为小麦改良的新种质来源。

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