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EST-SSR cross-amplification and genetic similarity in Onobrychis genus

机译:EST-SSR交叉扩增和Onobrychis属的遗传相似性

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摘要

EST-SSR from Medicago truncatula Gaertn. and Glycine max (L.) Merr. were tested for transferability in various species of Onobrychis (O. pyrenaica Sennen, O. argentea Boiss. and O. viciifolia Scop.). Repeatable amplification was obtained for 81% of the microsatellites and 52% were polymorphic. Six selected SSRs from M. truncatula were used to fingerprint and estimate the genetic similarity of a set of 23 accessions of O. viciifolia. PCA analysis discriminated among the different Onobrychis species and the sainfoin accessions were clustered in a single major group. This grouping is discussed in terms of the history of cultivation of sainfoin in Spain. The selected SSRs will allow fingerprinting and genetic studies in Onobrychis species, solving the lack of available SSR markers in this species.
机译:梅花苜蓿Gaertn的EST-SSR。和Glycine max(L.)Merr。在不同种类的Onobrychis(O. pyrenaica Sennen,O。argentea Boiss。和O. viciifolia Scop。)中测试了可转移性。 81%的微卫星可重复扩增,52%是多态的。从截短芒(M. truncatula)选出的6个SSR用于指纹图谱和估计23种短叶O.viciifolia品种的遗传相似性。 PCA分析在不同的Onobrychis物种之间进行了区分,并且皂甙成分被聚集在一个主要的群体中。根据西班牙皂素的种植历史对这一分组进行了讨论。选定的SSR将允许对Onobrychis物种进行指纹分析和遗传研究,从而解决该物种中缺乏可用的SSR标记的问题。

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