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首页> 外文期刊>Genetic Resources and Crop Evolution >Chromosome and genome composition of a Triticum x Thinopyrum hybrid by classical and molecular cytogenetic techniques
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Chromosome and genome composition of a Triticum x Thinopyrum hybrid by classical and molecular cytogenetic techniques

机译：Triticum x Thinopyrum杂种的经典基因和分子细胞遗传技术的染色体和基因组组成

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摘要

Triticum-Thinopyrum amphiploids arose from the need to obtain forage grasses highly resistant to pest, drought, soil salinity and frost and they can be used as efficient bridges to transfer desired genes from wheatgrass species to wheat. One of them is trigopiro SH16 INTA, it was introduced in Argentina in 1947 but its genomic composition was unknown. The aim of this work was to determine the chromosome number and genomic and chromosome composition of trigopiro SH16 INTA in order to use it in breeding programs. The simultaneous use of the in situ hybridization technique with different probes (genomic DNA of Th. ponticum (Podp.) Barkworth et D.R. Dewey, pSc119.2 and pAs1) allowed us to conclude that the chromosome number of trigopiro SH16 is 2n = 42 and the genome composition would be: 14 chromosomes of the B genome, the 2D and 4D chromosome pairs of wheat, 14 chromosomes of the J genome of Thinopyrum and the remaining chromosomes probably belong to the A genome of wheat.
机译：Triticum-Thinopyrum amploidloids是由于需要获得对病虫，干旱，土壤盐分和霜冻具有高度抵抗力的牧草而产生的，它们可以用作将所需基因从小麦草物种转移到小麦的有效桥梁。其中之一是trigopiro SH16 INTA，它于1947年在阿根廷被引入，但其基因组组成尚不清楚。这项工作的目的是确定trigopiro SH16 INTA的染色体数，基因组和染色体组成，以便将其用于育种程序。同时使用原位杂交技术和不同的探针（蓬皮草基因组DNA（Pokp。Barkworth等人，DR Dewey，pSc119.2和pAs1））可以推断出Trigopiro SH16的染色体数为2n = 42，基因组组成为：B基因组的14条染色体，小麦的2D和4D染色体对，Thinopyrum的J基因组的14条染色体和其余的染色体可能属于小麦的A基因组。

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