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Molecular marker diversity of SCN-resistant sources in soybean

机译：大豆抗SCN来源的分子标记多样性

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摘要

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe; HG) is one of the most destructive pests of soybean (Glycine max (L.) Merr.) in the United States. Over 100 SCN-resistant accessions within the USDA Soybean Germplasm Collection have been identified, but little is known about the genetic diversity of this SCN-resistant germplasm. The objective of this research was to evaluate the genetic variation and determine the genetic relationships among SCN-resistant accessions. One hundred twenty-two genotypes were evaluated by 85 simple sequence repeat (SSR) markers from 20 linkage groups. Non-hierarchical (VARCLUS) and hierarchical (Ward's) clustering were combined with multidimensional scaling (MDS) to determine relationships among tested lines. The 85 SSR markers produced 566 allelic fragments with a mean polymorphic information content (PIC) value of 0.35. The 122 lines were grouped into clusters by 2 different clustering methods and the MDS results consistently corresponded to the assigned clusters. Assigned clusters were dominated by genotypes that possess one or more unique SCN resistance genes and were associated with geographical origins. The results of analysis of molecular variance (AMOVA) showed that the variation differences among clusters and individual lines were significant, but the differences among individuals within clusters were not significant.
机译：大豆孢囊线虫（SCN）（Heterodera glycines Ichinohe; HG）是美国最具破坏力的大豆害虫之一（Glycine max（L.）Merr。）。在USDA大豆种质资源库中已鉴定出100多个抗SCN的种质，但对这种抗SCN的种质的遗传多样性知之甚少。这项研究的目的是评估遗传变异并确定SCN抗性种质之间的遗传关系。通过来自20个连锁组的85个简单序列重复（SSR）标记评估了122个基因型。将非分层（VARCLUS）和分层（Ward's）聚类与多维标度（MDS）结合起来，以确定测试线之间的关系。 85个SSR标记产生566个等位基因片段，平均多态信息含量（PIC）值为0.35。通过2种不同的聚类方法将122个品系分组为聚类，MDS结果始终与分配的聚类相对应。分配的簇以具有一个或多个独特的SCN抗性基因并与地理起源相关的基因型为主。分子方差分析（AMOVA）的结果表明，聚类和个体品系之间的变异差异是显着的，而聚类中个体之间的差异并不显着。

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