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首页> 外文期刊>Genome >Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH
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Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH

机译：五色BAC-FISH显示家蚕15号染色体与六倍体曼陀罗染色体之间的基因保守性

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摘要

The successful assignment of the existing genetic linkage groups (LGs) to individual chromosomes and the second-generation linkage map obtained by mapping a large number of bacterial artificial chromosome (BAC) contigs in the silkworm, Bombyx mori, together with public nucleotide sequence databases, offer a powerful tool for the study of synteny between karyotypes of B. mori and other lepidopteran species. Conserved synteny of genes between particular chromosomes can be identified by comparatively mapping orthologous genes of the corresponding linkage groups with the help of BAC-FISH (fluorescent in situ hybridization). This technique was established in B. mori for 2 differently labeled BAC probes simultaneously hybridized to pachytene bivalents. To achieve higher-throughput comparative mapping using BAC-FISH in Lepidoptera, we developed a protocol for five-color BAC-FISH, which allowed us to map simultaneously 6 different BAC probes to chromosome 15 in B. mori. We identified orthologs of 6 B. mori LG15 genes (RpP0, RpS8, eIF3, RpL7A, RpS23, and Hsc70) for the tobacco hornworm, Manduca sexta, and selected the ortholog-containing BAC clones from an M. sexta BAC library. All 6 M. sexta BAC clones hybridized to a single M. sexta bivalent in pachytene spermatocytes. Thus, we have confirmed the conserved synteny between the B. mori chromosome 15 and the corresponding M. sexta chromosome (hence provisionally termed chromosome 15).
机译：通过将家蚕Bombyx mori中的大量细菌人工染色体（BAC）重叠群与公共核苷酸序列数据库一起作图，将现有的遗传连锁组（LGs）成功分配给单个染色体，并获得第二代连锁图谱，为研究家蚕核型和其他鳞翅目物种的同态性提供了有力的工具。可以通过在BAC-FISH（荧光原位杂交）的帮助下对相应连接基团的直系同源基因进行作图来鉴定特定染色体之间基因的保守性。在家蚕中建立了该技术，用于同时与粗线烯二价杂交的2种不同标记的BAC探针。为了在鳞翅目中使用BAC-FISH实现更高通量的比较作图，我们开发了一种五色BAC-FISH的方案，该方案允许我们将6种不同的BAC探针同时映射到桑蚕中的15号染色体。我们确定了烟草角虫，曼杜卡六倍体的6个桑蚕LG15基因直系同源基因（RpP0，RpS8，eIF3，RpL7A，RpS23和Hsc70），并从M. sexta BAC文库中选择了含有直系同源基因的BAC克隆。所有的6个M. sexta BAC克隆都与上皮精子细胞中的单个M. sexta二价杂交。因此，我们已经证实了桑蚕芽孢杆菌15号染色体和相应的M. sexta染色体之间的保守同位（因此被暂时称为15号染色体）。

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