Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group (see comments)

Schnizlein Bick-CT; Spritzler J; Wilkening CLNicholson JKOGorman MR

首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group (see comments)

【24h】

Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group (see comments)

机译：

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A single-platform technology that uses an internal bead standard and three-color flow cytometry to determine CD4 and CD8 absolute counts was evaluated for reproducibility and agreement. Values obtained using TruCount absolute-count tubes were compared to those obtained using a two-color predicate methodology. Sixty specimens from human immunodeficiency virus type 1-infected donors were shipped to five laboratories. Each site also analyzed replicates of 14 human immunodeficiency virus type 1-infected local specimens at 6 h and again at 24 h. The interlaboratory variability was significantly less with TruCount (median difference in percent coefficient of variation CV between the two methods was -8 and -3 for CD4 and CD8, respectively) than with the predicate method. Intralaboratory variability was smaller, with a median difference in CV of -1 for both CD4 and CD8 with 6-h samples and -2 and -3 for CD4 and CD8, respectively, with 24-h samples. Use of TruCount for shipped samples resulted in a median CD4 count change of 7 cells (50th estimated percentile) when all laboratories and CD4 strata were combined. For on-site samples, the median CD4 count change was 10 CD4 cells for 6-h samples and 2 CD4 cells for 24-h samples. Individual site biases occurred in both directions and cancelled each other when the data were combined for all laboratories. Thus, the combined data showed a smaller change in median CD4 count than what may have occurred at an individual site. In summary, the use of TruCount decreased both the inter- and intralaboratory variability in determining absolute CD4 and CD8 counts.

著录项

来源
《Clinical and diagnostic laboratory immunology》 |2000年第3期|336-343|共8页
作者
Schnizlein Bick-CT; Spritzler J; Wilkening CLNicholson JKOGorman MR;
展开▼
作者单位

Department of Medicine/Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. cschnizl@iupui.edu;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类医学免疫学;诊断学;
关键词
Acquired Immunodeficiency Syndrome; CD4-CD8 Ratio; Flow Cytometry; HIV-1; 获得性免疫缺陷综合征; CD4-CD8比值; 流式细胞术;

相似文献

外文文献
中文文献

1. Predictive ability of Acute Physiology and Chronic Health Evaluation II scoring applied to human immunodeficiency virus-positive patients [J] . Michael C. MD Brown, William B. MD Crede critical care medicine . 1995,第5期

机译：Predictive ability of Acute Physiology and Chronic Health Evaluation II scoring applied to human immunodeficiency virus-positive patients
2. Proliferation of double‐negative (CD4−CD8−) T cells bearing T‐cell receptor‐αβ in a haemophiliac with human immunodeficiency virus type 1 infection and factor VIII inhibitor: functional properties of double‐negative T‐cell receptor‐αβ+T cells [J] . Masaki Yasukawa, Takaaki Hato, Mitsuru MatsumotoKiyonori TakadaShigeru FujitaYuzuru Kobayashi british journal of haematology . 1991,第3期

机译：Proliferation of double‐negative (CD4−CD8−) T cells bearing T‐cell receptor‐αβ in a haemophiliac with human immunodeficiency virus type 1 infection and factor VIII inhibitor: functional properties of double‐negative T‐cell receptor‐αβ+T cells
3. Broad, high-magnitude and multifunctional CD4(+) and CD8(+) T-cell responses elicited by a DNA and modified vaccinia Ankara vaccine containing human immunodeficiency virus type 1 subtype C genes in baboons [J] . Burgers WA, Chege GK, Muller TLvan Harmelen JHKhoury GShephard EGGray CMWilliamson CWilliamson AL The Journal of General Virology: A Federation of European Miorobiological Societies Journal . 2009,第2期

机译：Broad, high-magnitude and multifunctional CD4(+) and CD8(+) T-cell responses elicited by a DNA and modified vaccinia Ankara vaccine containing human immunodeficiency virus type 1 subtype C genes in baboons

Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group (see comments)

摘要

著录项

相似文献

相关主题

期刊订阅