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首页> 外文期刊>Fibers and Polymers >Highly Porous Three-dimensional Poly(lactide-co-glycolide) (PLGA) Microfibrous Scaffold Prepared by Electrospinning Method: a Comparison Study with Other PLGA Type Scaffolds on Its Biological Evaluation
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Highly Porous Three-dimensional Poly(lactide-co-glycolide) (PLGA) Microfibrous Scaffold Prepared by Electrospinning Method: a Comparison Study with Other PLGA Type Scaffolds on Its Biological Evaluation

机译：静电纺丝法制备的高孔隙度三维丙交酯-乙交酯共聚物（PLGA）微纤维支架：与其他PLGA型支架的生物学评价比较研究

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In this study, a three-dimensional (3D) poly(lactide-co-glycolide) (PLGA) microfibrous scaffold with high porosity (ca. 90 % porosity) was developed for evaluating its performance in tissue engineering application. A dope solution of PLGA/polyethylene oxide (PEO) blend was electrospun into a methanol coagulation bath for fabricating highly porous 3D PLGA scaffold and a salt leaching method was used for making interconnected pores of 100-200 μm size inside the scaffold. The morphological structure, pore size and porosity of the microfibrous scaffold were determined, and compared with two-dimensional (2D) mat-type and 3D sponge-type of PLGA scaffold. Also, swelling ratio, water uptake and compressive strength were compared in order to elucidate the structure-property relationships of different types of the scaffolds, especially in a wet condition. As a result of scanning electron microscopy (SEM) observation, normal human dermal fibroblasts (nHDF) were migrated, attached, and proliferated well inside the 3D scaffold. MTT assay confirmed that the highly porous 3D PLGA microfibrous scaffold had superior cell adhesion and proliferation abilities due to fibrous structure of large specific surface area, and interconnected pore structure. Therefore, this high performance 3D PLGA scaffold can have a high potentiality for application in tissue engineering in comparison with conventional PLGA scaffolds.
机译：在这项研究中，开发了一种具有高孔隙率（约90％孔隙率）的三维（3D）聚丙交酯-乙交酯共聚物（PLGA）微纤维支架，以评估其在组织工程应用中的性能。将PLGA /聚环氧乙烷（PEO）共混物的涂料溶液电纺到甲醇混凝浴中，以制造高度多孔的3D PLGA支架，并采用盐浸法在支架内部制作大小为100-200μm的互连孔。确定了微纤维支架的形态结构，孔径和孔隙率，并与二维（2D）垫型和3D海绵型PLGA支架进行了比较。另外，比较了溶胀率，吸水率和抗压强度，以阐明不同类型的支架的结构-性质关系，特别是在潮湿条件下。扫描电子显微镜（SEM）观察的结果是，正常的人类皮肤成纤维细胞（nHDF）在3D支架内迁移，附着和增殖良好。 MTT分析证实，由于大比表面积的纤维结构和相互连接的孔结构，高度多孔的3D PLGA微纤维支架具有优异的细胞粘附和增殖能力。因此，与传统的PLGA支架相比，这种高性能3D PLGA支架在组织工程中的应用具有很高的潜力。

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