Secretion of imunodominant membrane protien from onion yellows phytoplsma through the Sec protein-transolcation system in Escherichia coli

Shigeyuki kakizawa; Kenro Oshima; Hisashi NishigawaHee-Young JungWei WeiShiho SuzukiMinoru TanakaShin-ichi MiyataMasashi ugakiShigetou namba

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Secretion of imunodominant membrane protien from onion yellows phytoplsma through the Sec protein-transolcation system in Escherichia coli

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a gene that encodes a putative SecE protein,whichis a component of the Sec protein-transolcation system,was cloned from the onion yellows phytoplasma (OY).The identification of this gene and the previously reported genesencoding SecA and SecY provides evidence that the Sec system exists in phytoplasma.In addition,a gene encodingan antigenic membrane protein(Amp) (a type of immunodominant membraneprotein) of OY was cloned and sequenced.The OY amp gene consisted of 702 nt encodinga protein of 233 aa which was highly similar to Amp of aster yellows phytoplasma(AY).Part of OY Amp was overexpressed in Escherichia coli,purified,and used to raise an anti-Amp polyclonal antibody.the anti-Amp antibody reacted specifically with an OY-infected plant extract in Western blot analysis and was therefore useful exported by the Sec system of E.coli.A partial OY Amp protein expressed in E.coli was localized in the periplasm as a shorter,putatively processed form of the protein.It had probably been exported from the cytoplasm to the periplasm through the Sec system.Morevover,OY Amp protein expressed in OY anddetected in OY-infected plants was apparentlyalsoprocessed.because phytoplasmas cannot becultured or transsformed,little information is available regarding their protein secretion systems.This study suggests that the Sec system operates in this phytoplasma to exprot OY Amp.

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来源
《Microbiology》 |2004年第1期|135-142|共8页
作者
Shigeyuki kakizawa; Kenro Oshima; Hisashi NishigawaHee-Young JungWei WeiShiho SuzukiMinoru TanakaShin-ichi MiyataMasashi ugakiShigetou namba;
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1Division of Integrated Biosciences,The University of Tokyo,202 Bioscience Bldg,5-1-5 kashiwanoha,Chiba 277-8562,Japan;

Division of Agricultura and Life Sciences,The University of Tokyo,1-1-1 Yayoi,Bunkyo-ku,Tokyo 113-8657,Japan;

1Division of Integrated Biosciences,The University of Tokyo,202 Bioscience Bldg,5-1-5 kashiwanoha,Chiba 277-8562,JapanDivision of Agricultura and Life Sciences,The University of Tokyo,1-1-1 Yayoi,Bunkyo-ku,Tokyo 113-8657,JapanNationa Agricultural ResearchCenter,National Agricultura Research organization 3-1-1 Kannondai,Tsukuba-shi,Ibaraki 305-8666,Japan1Division of Integrated Biosciences,The University of Tokyo,202 Bioscience Bldg,5-1-5 kashiwanoha,Chiba 277-8562,Japan.Division of Agricultura and Life Sciences,The University of Tokyo,1-1-1 Yayoi,Bunkyo-ku,Tokyo 113-8657,Japan;

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中图分类微生物学;
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Secretion of imunodominant membrane protien from onion yellows phytoplsma through the Sec protein-transolcation system in Escherichia coli

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