Kruppel-like factor 4 prevents centrosome amplification following gamma-irradiation-induced DNA damage.

Yoon HS; Ghaleb AM; Nandan MOHisamuddin IMDalton WBYang VW

首页> 外文期刊>Oncogene >Kruppel-like factor 4 prevents centrosome amplification following gamma-irradiation-induced DNA damage.

【24h】

Kruppel-like factor 4 prevents centrosome amplification following gamma-irradiation-induced DNA damage.

机译：

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Centrosome duplication is a carefully controlled process in the cell cycle. Previous studies indicate that the tumor suppressor, p53, regulates centrosome duplication. Here, we present evidence for the involvement of the mammalian Kruppel-like transcription factor, KLF4, in preventing centrosome amplification following DNA damage caused by gamma-irradiation. The colon cancer cell line HCT116, which contains wild-type p53 alleles (HCT116 p53+/+), displayed stable centrosome numbers following gamma-irradiation. In contrast, HCT116 cells null for the p53 alleles (HCT116 p53-/-) exhibited centrosome amplification after irradiation. In the latter cell line, KLF4 was not activated following gamma-irradiation due to the absence of p53. However, centrosome amplification could be suppressed in irradiated HCT116 p53-/- cells by conditional induction of exogenous KLF4. Conversely, in a HCT116 p53+/+ cell line stably transfected with small hairpin RNA (shRNA) designed to specifically inhibit KLF4, gamma-irradiation induced centrosome amplification. In these cells, the inability of KLF4 to become activated in response to DNA damage was directly associated with an increase in cyclin E level and Cdk2 activity, both essential for regulating centrosome duplication. Cotransfection experiments showed that KLF4 overexpression suppressed the promoter activity of the cyclin E gene. The results of this study demonstrated that KLF4 is both necessary and sufficient in preventing centrosome amplification following gamma-radiation-induced DNA damage and does so by transcriptionally suppressing cyclin E expression.

著录项

来源
《Oncogene》 |2005年第25期|4017-4025|共9页
作者
Yoon HS; Ghaleb AM; Nandan MOHisamuddin IMDalton WBYang VW;
展开▼
作者单位

Division of Digestive Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类肿瘤学;
关键词
Centrosome; DNA Damage; DNA-Binding Proteins; Gamma Rays; Transcription Factors; 中心体; DNA损伤; DNA结合蛋白质类; γ射线; 转录因子;

相似文献

外文文献
中文文献
专利

1. A global DNA repair mechanism involving the Cockayne syndrome B (CSB) gene product can prevent the in vivo accumulation of endogenous oxidative DNA base damage. [J] . Osterod M, Larsen E, Le Page FHengstler JGVan Der Horst GTBoiteux SKlungland AEpe B Oncogene . 2002,第54期

机译：A global DNA repair mechanism involving the Cockayne syndrome B (CSB) gene product can prevent the in vivo accumulation of endogenous oxidative DNA base damage.
2. Expression of the gut-enriched Kruppel-like factor (Kruppel-like factor 4) gene in the human colon cancer cell line RKO is dependent on CDX2. [J] . Dang DT, Mahatan CS, Dang LHAgboola IAYang VW Oncogene . 2001,第35期

机译：Expression of the gut-enriched Kruppel-like factor (Kruppel-like factor 4) gene in the human colon cancer cell line RKO is dependent on CDX2.
3. DNA-PK/Chk2 induces centrosome amplification during prolonged replication stress [J] . Wang C-Y, Huang E. Y-H, Huang S-cChung B-c Oncogene . 2015,第10期

机译：DNA-PK/Chk2 induces centrosome amplification during prolonged replication stress
4. Erythroid Kruppel-like factor (EKLF) is recruited to the γ-globin gene promoter as a co-activator and is required for γ-globin gene induction by short-chain fatty acid derivatives [O] . Susan P. Perrine, Rishikesh Mankidy, Michael S. Boosalis, -1

机译：Erythroid Kruppel-like factor（EKLF）被募集到γ-珠蛋白基因启动子中作为共激活因子并且是短链脂肪酸衍生物诱导γ-珠蛋白基因所必需的
5. Kruppel-Like Factor 2-Mediated Suppression of MicroRNA-155 Reduces the Proinflammatory Activation of Macrophages. [O] . Shaolin He, Liyuan Yang, Dazhu Li, 2015

机译：Kruppel-Like Factor 2介导的microRNa-155抑制可减少巨噬细胞的促炎活化。

Kruppel-like factor 4 prevents centrosome amplification following gamma-irradiation-induced DNA damage.

摘要

著录项

相似文献

相关主题

期刊订阅