Octabromo-tetrakis(4-methylpyridyl)porphine (OBTMPyP), an octabromonated compound with 4 pyrole rings of tetrakis(4-methylpyridyl)porphine, selectively forms a complex with Cu 2+ ions at pH 2.0. When 3.6×10 −5 mol/L OBTMPyP was added to the reaction mixture, the calibration curve showed good linearity for Cu 2+ ions ranging from 0.01-2.2 µg (addition of 1.0 mL). A good coefficient of variation (Cu 2+ ions=1.5 µg (addition of 1.0 mL), n =10, 0.8) was obtained. The molar absorption coefficient (ε) based on Cu 2+ ions was 8.5×10 4 L/mol•cm. This value was 6-fold greater than that determined with a clinical chemical analysis kit using the bathocuproine sulfonic acid method, which is a well-known method for spectrophotometric determination of the Cu 2+ ion concentration. A deproteination method was successfully applied in the clinical analysis kit for determination of Cu 2+ ion concentrations in control serum I, and the values determined using this method and the bathocuproine sulfonic acid method were almost the same.
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