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Ceriofast™: An acute toxicity test based onCeriodaphnia dubiafeeding behavior

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AbstractWe have developed a rapid acute toxicity test (CerioFAST™) based on suppression of feeding activity ofCeriodaphnia dubiain the presence of toxicants. The bioassay consists of a 1‐h exposure period to a given toxicant. Yeast cells, stained with a fluorescent dye, are added 20 min before the end of the exposure period. Response to a toxic sample is indicated by the absence of fluorescence in the gut of the daphnids. CerioFAST was compared to the standard 48‐hC. dubiaacute bioassay, using heavy metals and organic compounds. CerioFAST EC50s of Cd, Cu, Pb, Ag, Zn, and carbofuran were in the 0.01–0.1‐mg/L range, whereas EC50s of hexachloroethane, pentachlorophenol, trichlorophenol, and lindane were in the 1–10‐mg/L range. CerioFAST EC50s of the heavy metals and organics were well correlated with EC50s obtained with the 48‐hC

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