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首页> 外文期刊>Fisheries and Aquatic Sciences >Toxicity of Cryoprotectants to Gametophytic Thalli of Red Algae Porphyra yezoensis
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Toxicity of Cryoprotectants to Gametophytic Thalli of Red Algae Porphyra yezoensis

机译:低温保护剂对红藻紫菜配子体生殖器官的毒性

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摘要

We assessed the toxicity of cryoprotectant agents (CPAs) to gametophytic thalli of red alga Porphyra yezoensis at room temperature. The CPAs used were: dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol (GC), 1,2-butanediol (1,2-BD), 1,3-bu-tanediol (1,3-BD), 2,3-butanediol (2,3-BD), 1,3-propanediol (1,3-PD) and propylene glycol (PG). CPA concentrations of 10, 15, 20, 25, 30, 35, 40, 45, and 50% were employed with 30 or 60 s immersion. The toxicity of the eight CPAs to gametophytic thalli of P. yezoensis was in the order: 1,3-BD < DMSO approx= 2,3-BD approx= PG approx= EG < GC < 1,3-PD approx= 1,2-BD. All thalli were more sensitive to high CPA concentrations, and most (>75%) thalli survived exposure to 10-25% CPA for 60 s. These data will facilitate selection of the optimal cryoprotectant concentration for cryopreservation of P. yezoensis thalli.
机译:我们在室温下评估了冷冻保护剂(CPA)对赤藻紫菜的配子体thalli的毒性。使用的CPA是:二甲基亚砜(DMSO),乙二醇(EG),甘油(GC),1,2-丁二醇(1,2-BD),1,3-丁二醇(1,3-BD), 2,3-丁二醇(2,3-BD),1,3-丙二醇(1,3-PD)和丙二醇(PG)。 CPA浓度为10%,15%,20%,25%,30%,35%,40%,45%和50%,浸入时间为30或60 s。八种CPA对yezoensis配子体thalli的毒性顺序为:1,3-BD 75%)thalli在暴露于10-25%CPA的条件下存活了60 s。这些数据将有助于选择最佳的冷冻保护剂浓度,以用于冻存P. yezoensis thalli。

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