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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Characterization of a Novel Alkali-Solyble Heteropolysaccharide from Tetraplold Gynostemma pentaphyllum Makino and Its Potential Anti-inflammatory and Antioxidant Properties
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Characterization of a Novel Alkali-Solyble Heteropolysaccharide from Tetraplold Gynostemma pentaphyllum Makino and Its Potential Anti-inflammatory and Antioxidant Properties

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摘要

A novel polysaccharide (GPP-S), with a molecular mass of 1 2 X 106 Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19,49.7 82- The structural analysis suggested that the backbone of GPP-S is (1->4)~linked-gIucose and (1->6)-linked-galactose with a (1->4,6)-linked-glucose branch every six monosaccharide residues The terminals were 1-)-oarabmose, glucuronic acid, and other monosaccharides GPP-S exhibited scavenging capacities against hydroxyl, peroxyl, and DPPH~~R radicals in vitro, GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells These results suggested that GPP-S could be developed as a bioactive ingredient for functional foods and dietary supplements

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