Comparative expressed sequence hybridization to chromosomes for tumor classification and identification of genomic regions of differential gene expression.

Lu YJ; Williamson D; Clark JWang RTiffin NSkelton LGordon TWilliams RAllan BJackman ACooper CPritchard-Jones KShipley J

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Comparative expressed sequence hybridization to chromosomes for tumor classification and identification of genomic regions of differential gene expression.

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Altered expression of genes can have phenotypic consequences in cancer development and treatment, developmental abnormalities, and differentiation processes. Here we describe a rapid approach, termed comparative expressed sequence hybridization (CESH), which gives a genome-wide view of relative expression patterns within tissues according to chromosomal location. No prior knowledge of genes or cloning is required, and minimal amounts of tissue can be used. Expression profiles are achieved in a manner similar to the identification of chromosomal imbalances by comparative genomic hybridization analysis. The approach is demonstrated to indicate a chromosomal region that harbors overexpressed genes that may be associated with a drug-resistant phenotype. In addition, known and new regions of differential gene expression in both normal tissues and tumor samples from the soft tissue sarcoma group of rhabdomyosarcoma (RMS) are indicated. These regions included 2p24; overexpression of MYCN at 2p24 was confirmed by quantitative reverse transcription-PCR for all of the alveolar RMS cases and did not necessarily correspond to genomic amplification. Evidence including region specific microarray analysis indicated that overexpression of several genes from a region may be required for detection by CESH. This evidence is consistent with clusters of functionally related genes and mechanisms that affect the expression of a number of genes at a particular genomic location. The distinctive CESH profiles demonstrated in different subtypes of RMS show potential for tumor classification.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第16期|9197-9202|共6页
作者
Lu YJ; Williamson D; Clark JWang RTiffin NSkelton LGordon TWilliams RAllan BJackman ACooper CPritchard-Jones KShipley J;
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作者单位

Molecular Cytogenetics, Institute of Cancer Research, Sutton, Surrey SM2 5NG, United Kingdom.;

hylo.life.uiuc.edu;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Chromosomes; Human; Gene Expression; Genome; Yeast Artificial; 染色体; 人; 基因表达; 基因组;

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Comparative expressed sequence hybridization to chromosomes for tumor classification and identification of genomic regions of differential gene expression.

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