Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition.

Bauer S; Kirschning CJ; Hacker HRedecke VHausmann SAkira SWagner HLipford GB

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Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition.

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摘要

The Toll-like receptor (TLR) family consists of phylogenetically conserved transmembrane proteins, which function as mediators of innate immunity for recognition of pathogen-derived ligands and subsequent cell activation via the Toll/IL-1R signal pathway. Here, we show that human TLR9 (hTLR9) expression in human immune cells correlates with responsiveness to bacterial deoxycytidylate-phosphate-deoxyguanylate (CpG)-DNA. Notably "gain of function" to immunostimulatory CpG-DNA is achieved by expressing TLR9 in human nonresponder cells. Transfection of either human or murine TLR9 conferred responsiveness in a CD14- and MD2-independent manner, yet required species-specific CpG-DNA motifs for initiation of the Toll/IL-1R signal pathway via MyD88. The optimal CpG motif for hTLR9 was GTCGTT, whereas the optimal murine sequence was GACGTT. Overall, these data suggest that hTLR9 conveys CpG-DNA responsiveness to human cells by directly engaging immunostimulating CpG-DNA.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第16期|9237-9242|共6页
作者
Bauer S; Kirschning CJ; Hacker HRedecke VHausmann SAkira SWagner HLipford GB;
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作者单位

Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, 81675 Munich, Germany. stefan.bauer;

hylo.life.uiuc.edu;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
CpG Islands; DNA; Bacterial; DNA-Binding Proteins; Receptors; Cell Surface; CpG岛; 细菌; DNA结合蛋白质类; 受体; 细胞表面;

Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition.

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