Identification of bifunctional Delta 12/omega 3 fatty acid desaturases for improving the ratio of omega 3 to omega 6 fatty acids in microbes and plants

Damude  HG; Zhang  HX; Farrall  LRipp  KGTomb  JFHollerbach  DYadav  NS

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Identification of bifunctional Delta 12/omega 3 fatty acid desaturases for improving the ratio of omega 3 to omega 6 fatty acids in microbes and plants

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We report the identification of bifunctional Delta 12/omega 3 desaturases from Fusarium moniliforme, Fusarium graminearum, and Magnaporthe grisea. The bifunctional activity of these desaturases distinguishes them from all known Delta 12 or omega 3 fatty acid desaturases. The omega 3 desaturase activity of these enzymes also shows a broad omega 6 fatty acid substrate specificity by their ability to convert linoleic acid (LA), gamma-linolenic acid, di-homo-gamma-linolenic acid, and arachidonic acid to the omega 3 fatty acids, alpha-linolenic acid (ALA), stearidonic acid, eicosatetraenoic acid, and eicosapentaenoic acid (EPA), respectively. Phylogenetic analysis suggests that omega 3 desaturases arose by independent gene duplication events from a Delta 12 desaturases ancestor. Expression of F. moniliforme Delta 12/omega 3 desaturase resulted in high ALA content in both Yarrowia lipolytica, an oleaginous yeast naturally deficient in omega 3 desaturation, and soybean. In soybean, seed-specific expression resulted in 70.9 weight percent of total fatty acid (TFA) ALA in a transformed seed compared with 10.9TFA in a null segregant seed and 53.2TFA in the current best source of ALA, linseed oil. The ALA/LA ratio in transformed seed was 22.3, a 110- and 7-fold improvement over the null segregant seed and linseed oil, respectively. Thus, these desaturases have potential for producing nutritionally desirable omega 3 long-chain polyunsaturated fatty acids, such as EPA, with a significantly improved ratio of omega 3/omega 6 long-chain polyunsaturated fatty acids in both oilseeds and oleaginous microbes.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2006年第25期|9446-9451|共6页
作者
Damude HG; Zhang HX; Farrall LRipp KGTomb JFHollerbach DYadav NS;
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作者单位

DuPont Co Inc, DuPont Expt Stn, Cent Res & Dev, Wilmington, DE 19898 USA;

DuPont Co Inc, DuPont Expt Stn, Wilmington, DE 19880 USA;

Unitat de Quimica Analitica,Department de Quimica,University Autonoma de Barcelona,Bellaterra,Spain;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
polyunsaturated fatty acids; Yarrowia; soybean; YEAST YARROWIA-LIPOLYTICA; SACCHAROMYCES-CEREVISIAE; TRANSGENIC PLANTS; EICOSAPENTAENOIC ACID; SUBSTRATE-SPECIFICITY; BIOSYNTHESIS; EXPRESSION; OILS; CRYPTOREGIOCHEMISTRY; ARABIDOPSIS;

Identification of bifunctional Delta 12/omega 3 fatty acid desaturases for improving the ratio of omega 3 to omega 6 fatty acids in microbes and plants

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