Update of pneumococcal PCR serotyping assay for detection of a commonly occurring type 19F wzy variant in Brazil

DeO.MenezesA.P.; ReisJ.N.; TernesY.M.AndradeA.L.PimentaF.C.DaGloriaCarvalhoM.BeallB.

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Update of pneumococcal PCR serotyping assay for detection of a commonly occurring type 19F wzy variant in Brazil

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In this era of effective pneumococcal conjugate vaccines, simple and inexpensive methods are desirable for determining capsular serotype (st) distributions. Although a multiplexed conventional PCR (cmPCR) approach is generally reliable (1-4) (updates at www.cdc.gov/ncidod/biotech/strep/pcr.htm), the key serotypes 19A and 19F have presented problems (5, 6). The wzy19A- and wzy19F-encoded oligosaccharide repeat unit polymerases puta-tively provide the basis of the structural difference between the stl9A and stl9F capsules (7). Recently, we reported an stl9F isolate recovered in Canada (strain 2584-0819F) that yielded a false-positive cmPCR type 19A (cmPCR-19A) result and a false-negative cmPCR-19F result (5). We redesigned our stl9A primers in order to avoid further false-positive PCR-19As.

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《Journal of Clinical Microbiology》 |2013年第7期|2470-2471|共2页
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DeO.MenezesA.P.; ReisJ.N.; TernesY.M.AndradeA.L.PimentaF.C.DaGloriaCarvalhoM.BeallB.;
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Centro de Pesquisas Gon?alo Moniz, Funda??o Oswaldo Cruz, Salvador, Bahia, Brazil;

Centro de Pesquisas Gon?alo Moniz, Funda??o Oswaldo Cruz, Salvador, Bahia, Brazil, Faculdade de;

Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania, BrazilDivision of Bacterial Disease, Respiratory Diseases Branch, Centers for Disease Control and;

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正文语种英语
中图分类医学微生物学（病原细菌学、病原微生物学）;
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Update of pneumococcal PCR serotyping assay for detection of a commonly occurring type 19F wzy variant in Brazil

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