Identification of a detection panel for post-transplant virus infection through integrated analysis of non-coding RNAs in peripheral blood

Huadi Chen; Anli Yang; Chenglin WuJianwei LinXiaoping WangMengran PengDian LiTao ZhangQiang ZhaoXiaoshun He

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Identification of a detection panel for post-transplant virus infection through integrated analysis of non-coding RNAs in peripheral blood

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Viral infection seriously affects the survival and life quality of transplanted patients without an accurate diagnosis during the early stage. Herein, we aimed to develop a novel diagnostic method based on non-coding RNAs expression in peripheral blood. An immunosuppressive mouse model of viral infection after transplantation was established. Differentially expressed non-coding RNAs were distinguished by microarray analyses in the virus-infected group. After homology analysis, 46 miRNAs and 24 lncRNAs were further verified by qRT-PCR in the peripheral blood samples of transplanted patients. Compared with normal transplanted patients, miR-29b, miR-185, and NR_073415.2 were significantly downregulated in the PBMC of post-transplant patients with viral infection. Based on the expression of the above three RNAs, principal component analysis (PCA) identified a slight overlap between the two groups. A 3-non-coding-RNA detection panel was constructed by the support vector machine analysis (SVM), whose loss rate was 14.71. The area under the curve of it was 0.909. With the optimal cut-off value ( Y = 0.328), the sensitivity was 0.929 and the specificity was 0.781. Therefore, based on non-coding RNAs expressions, a detection panel for viral infection after organ transplantation was formed with high diagnostic specificity and sensitivity.

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《Artificial cells, nanomedicine, and biotechnology.》 |2021年第1期|691-698|共8页
作者
Huadi Chen; Anli Yang; Chenglin WuJianwei LinXiaoping WangMengran PengDian LiTao ZhangQiang ZhaoXiaoshun He;
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Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University;

Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology;

Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation)Department of Breast Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer MedicineDepartment of Hepatobiliary and Pancreas Surgery, Shenzhen People's HospitalDermatology Department, The Third Affiliated Hospital of Sun Yat-sen UniversityDepartment of Data Science, Dana Farber Cancer Institute, Harvard School of Public Health;

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Viral infection; diagnosis; transplantation; non-coding RNA; miR-29b; miR-185; NR_073415.2;

Identification of a detection panel for post-transplant virus infection through integrated analysis of non-coding RNAs in peripheral blood

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