Aragonite crystallization in primary cell cultures of multicellular isolates from a hard coral, Pocillopora damicornis.

Domart-Coulon IJ; Elbert DC; Scully EPCalimlim PSOstrander GK

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Aragonite crystallization in primary cell cultures of multicellular isolates from a hard coral, Pocillopora damicornis.

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The foundation of marine coral reef ecosystems is calcium carbonate accumulated primarily by the action of hard corals (Coelenterata: Anthozoa: Scleractinia). Colonial hard coral polyps cover the surface of the reef and deposit calcium carbonate as the aragonite polymorph, stabilized into a continuous calcareous skeleton. Scleractinian coral skeleton composition and architecture are well documented; however, the cellular mechanisms of calcification are poorly understood. There is little information on the nature of the coral cell types involved or their cooperation in biocalcification. We report aragonite crystallization in primary cell cultures of a hard coral, Pocillopora damicornis. Cells of apical coral colony fragments were isolated by spontaneous in vitro dissociation. Single dissociated cell types were separated by density in a discontinuous Percoll gradient. Primary cell cultures displayed a transient increase in alkaline phosphatase (ALP) activity, to the level observed in intact corals. In adherent multicellular isolate cultures, enzyme activation was followed by precipitation of aragonite. Modification of the ionic formulation of the medium prolonged maintenance of isolates, delayed ALP activation, and delayed aragonite precipitation. These results demonstrate that in vitro crystallization of aragonite in coral cell cultures is possible, and provides an innovative approach to investigate reef-building coral calcification at the cellular level.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第21期|11885-11890|共6页
作者
Domart-Coulon IJ; Elbert DC; Scully EPCalimlim PSOstrander GK;
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作者单位

Department of Biology and Division of Comparative Medicine, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Calcium Carbonate; Cnidaria; 碳酸钙; 腔肠动物门;

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Aragonite crystallization in primary cell cultures of multicellular isolates from a hard coral, Pocillopora damicornis.

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