Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.

Pavlov YI; Galkin AP; Hanaoka FKunkel TA

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.

【24h】

Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.

机译：

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

To test the hypothesis that inaccurate DNA synthesis by mammalian DNA polymerase eta (pol eta) contributes to somatic hypermutation (SHM) of Ig genes, we measured the error specificity of mouse pol eta during synthesis of each strand of a mouse Ig kappa light chain transgene. We then compared the results to the base substitution specificity of SHM of this same gene in the mouse. The in vitro and in vivo base substitution spectra shared a number of common features. A highly significant correlation was observed for overall substitutions at A-T pairs but not for substitutions at G-C pairs. Sixteen mutational hotspots at A-T pairs observed in vivo were also found in spectra generated by mouse pol eta in vitro. The correlation was strongest for errors made by pol eta during synthesis of the non-transcribed strand, but it was also observed for synthesis of the transcribed strand. These facts, and the distribution of substitutions generated in vivo, support the hypothesis that pol eta contributes to SHM of Ig genes at A-T pairs via short patches of low fidelity DNA synthesis of both strands, but with a preference for the non-transcribed strand.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2002年第15期|9954-9959|共6页
作者
Pavlov YI; Galkin AP; Hanaoka FKunkel TA;
展开▼
作者单位

Laboratories of Molecular Genetics and Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709;

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090,;

zh.gov.pl;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Laboratory mice; Base; NOS; in vivo; Rad30 protein;

相似文献

外文文献
中文文献

1. Complementary Roles of Antibody Heavy and Light Chain Somatic Hypermutation in Conferring Breadth and Potency to the HIV-1-Specific CAP256-VRC26 bNAb Lineage [J] . Sacks David, Wiehe Kevin, Morris LynnMoore Penny L. Journal of Virology . 2022,第10期

机译：Complementary Roles of Antibody Heavy and Light Chain Somatic Hypermutation in Conferring Breadth and Potency to the HIV-1-Specific CAP256-VRC26 bNAb Lineage
2. Somatic mutation and CDR3 lengths of immunoglobulin kappa light chains expressed in patients with rheumatoid arthritis and in normal individuals. [J] . Bridges SL Jr, Lee SK, Johnson MLLavelle JCFowler PGKoopman WJSchroeder HW Jr The Journal of Clinical Investigation: The Official Journal of the American Society for Clinical Investigation . 1995,第2期

机译：Somatic mutation and CDR3 lengths of immunoglobulin kappa light chains expressed in patients with rheumatoid arthritis and in normal individuals.
3. Mass spectrometric‐based assessment of the serum kappa to lambda immunoglobulin light chain ratio (κ:λ) in dogs with immunoglobulin secretory diseases [J] . A Russell Moore, Kitty Brown, Cecelia ChapmanCorey Broeckling Veterinary and Comparative Oncology . 2023,第3期

机译：Mass spectrometric‐based assessment of the serum kappa to lambda immunoglobulin light chain ratio (κ:λ) in dogs with immunoglobulin secretory diseases

Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.

摘要

著录项

相似文献

相关主题

期刊订阅