Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

Yang D; Buchholz F; Huang ZGoga AChen CYBrodsky FMBishop JM

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

【24h】

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

机译：

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing capacities, RNA interference with synthetic siRNA is inefficient and cost intensive, especially for functional genomic studies. Here we report the use of Escherichia coli RNase III to cleave double-stranded RNA (dsRNA) into endoribonuclease-prepared siRNA (esiRNA) that can target multiple sites within an mRNA. esiRNA recapitulates the potent and specific inhibition by long dsRNA in Drosophila S2 cells. In contrast to long dsRNA, esiRNA mediates effective RNA interference without apparent nonspecific effect in cultured mammalian cells. We found that sequence-specific interference by esiRNA and the nonspecific IFN response activated by long dsRNA are independent pathways in mammalian cells. esiRNA works by eliciting the destruction of its cognate mRNA. Because of its simplicity and potency, this approach is useful for analysis of mammalian gene functions.

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2002年第15期|9942-9947|共6页
作者
Yang D; Buchholz F; Huang ZGoga AChen CYBrodsky FMBishop JM;
展开▼
作者单位

G. W. Hooper Foundation and Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0552, USA. dyang20;

ol.net;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Endoribonucleases; Escherichia coli; Gene Silencing; Nucleic Acid Heteroduplexes; 内切核糖核酸酶类; 大肠杆菌; 核酸异源双链分子; RNA; 双链;

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

摘要

著录项

相关主题

期刊订阅