Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites.

Dickson L; Huang HR; Liu LMatsuura MLambowitz AMPerlman PS

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Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites.

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Group II introns, the presumed ancestors of nuclear pre-mRNA introns, are site-specific retroelements. In addition to "homing" to unoccupied sites in intronless alleles, group II introns transpose at low frequency to ectopic sites that resemble the normal homing site. Two general mechanisms have been proposed for group II intron transposition, one involving reverse splicing of the intron RNA directly into an ectopic DNA site, and the other involving reverse splicing into a site in RNA followed by reverse transcription and integration of the resulting cDNA by homologous recombination. Here, by using an "inverted-site" strategy, we show that the yeast mtDNA group II intron aI1 retrotransposes by reverse splicing directly into an ectopic DNA site. This same mechanism could account for other previously described ectopic transposition events in fungi and bacteria and may have contributed to the dispersal of group II introns into different genes.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第23期|13207-13212|共6页
作者
Dickson L; Huang HR; Liu LMatsuura MLambowitz AMPerlman PS;
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作者单位

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9148, USA.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
DNA; Fungal; Introns; Retroelements; Saccharomyces cerevisiae; 真菌; 内含子; 反转录因子; 酵母菌; 酿酒;

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1. RNA splicing in yeast mitochondria: DNA sequence analysis ofmit−mutants deficient in the excision of introns aI1 and aI2 of the gene for subunit I of cytochrome c oxidase [J] . R.van derVeen, M.deHaan, L.A.Grivell current genetics . 1988,第3期

机译：RNA splicing in yeast mitochondria: DNA sequence analysis ofmit−mutants deficient in the excision of introns aI1 and aI2 of the gene for subunit I of cytochrome c oxidase
2. A multitude of suppressors of group II intron-splicing defects in yeast [J] . MartinWaldherr, AntonellaRagnini, BernhardJankRomanTeplyGerlindeWiesenbergerRudolfJ.Schweyen current genetics . 1993,第4期

机译：A multitude of suppressors of group II intron-splicing defects in yeast
3. GC content, but not nucleosome positioning, directly contributes to intron splicing efficiency in Paramecium [J] . Gnan Stefano, Matelot Melody, Weiman MarionArnaiz OlivierGuerin FredericSperling LindaBetermier MireilleThermes ClaudeChen Chun-LongDuharcourt Sandra Genome research . 2022,第4期

机译：GC content, but not nucleosome positioning, directly contributes to intron splicing efficiency in Paramecium

Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites.

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