Gene inactivation by multiphoton-targeted photochemistry.

Berns MW; Wang Z; Dunn AWallace VVenugopalan V

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Gene inactivation by multiphoton-targeted photochemistry.

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Multiphoton-targeted photochemistry was used to selectively inactivate the expression of genes in vertebrate cells. A membrane permeable DNA-associating vital dye, ethidium bromide monoacetate (visible wavelength single photon absorption peak at 530 nm) was used to photosensitize chromosomes in dividing cells. A 100-ps infrared laser beam operating at 1.06 microns was focused onto a selected region of a mitotic chromosome corresponding to the sites of the nucleolar (ribosomal) genes. Individual cells followed through mitosis demonstrated a reduction in the number of nucleoli formed in daughter cells that corresponded to the number of nucleolar genes sites irradiated. These results demonstrate the ability to selectively manipulate genes by using the focal point specificity characteristic of multiphoton microscopy. This technique should have wide biotechnology applications both in vitro and in vivo.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2000年第17期|9504-9507|共4页
作者
Berns MW; Wang Z; Dunn AWallace VVenugopalan V;
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作者单位

Beckman Laser Institute and Medical Clinic and Center for Biomedical Engineering, University of California, Irvine 92612-1475, USA. mberns@bli.uci.edu;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Biotechnology; Gene Silencing radiation effects; Genes; Photochemistry; Photons; 生物技术; 基因; 光化学; 光子;

Gene inactivation by multiphoton-targeted photochemistry.

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