Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells.

Christian AT; Pattee MS; Attix CMReed BESorensen KJTucker JD

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Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells.

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摘要

Rolling circle amplification has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate that rolling circle amplification in situ can detect gene copy number and single base mutations in fixed cells with efficiencies up to 90. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2001年第25期|14238-14243|共6页
作者
Christian AT; Pattee MS; Attix CMReed BESorensen KJTucker JD;
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作者单位

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551, USA. christian4;

fizer.com;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
DNA; Point Mutation; RNA; Messenger; 点突变; 信使;

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Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells.

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