Pathway Dependent Isotopic Fractionation during Aerobic Biodegradation of 1,2-Dichloroethane

Sarah K.Hirschorn; M.Joyce Dinglasan; Martin ElsnerSilvia A.ManciniGeorges Lacrampe-CouloumeElizabeth A.EdwardsBarbara Sherwood Lollar

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Pathway Dependent Isotopic Fractionation during Aerobic Biodegradation of 1,2-Dichloroethane

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1,2-Dichloroethane (1,2-DCA) is a widespread groundwater contaminant known to be biodegradable under aerobic conditions via enzymatic oxidation or hydrolytic dehaloge-nation reactions.Current literature reports that stable carbon isotope fractionation of 1,2-DCA during aerobic biodegradation is large and reproducible (-27 to -33per thousand).In this study,a significant variation in the magnitude of stable carbon isotope fractionation during aerobic biodegradation was observed.Biodegradation in experiments involving microcosms,enrichment cultures,and pure microbial cultures produced a consistent bimodal distribution of enrichment factors (e) with one mean e centered on -3.9+-0.6per thousand and the other on -29.2+-1.9per thousand.Reevaluation of epsilon in terms of kinetic isotope effects ~(12)kappa/~(13)kappa gave values of ~(12)kappa/~(13)kappa=1.01 and 1.06,which are typical of oxidation and hydrolytic dehalogenation (S_N2) reactions,respectively.The bimodal distribution is therefore consistent with the microbial degradation of 1,2-DCA by two separate enzymatic pathways.This interpretation is further supported in this study by experiments with pure strains of Xanthobacterautotrophicus GJ10,Ancylobacter aquaticus AD20,and Pseudomonas sp.Strain DCA1 for which the enzymatic degradation pathways are well-known.A small fractionation of -3.0per thousand was measured for 1,2-DCA degradation by Pseudomonas sp.Strain DCA1 (monooxygenase enzyme),while degradation by the hydrolytic dehalogenase enzyme by the other two pure strains was characterized by fractionation of -32.3per thousand.

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《Environmental Science & Technology: ES&T》 |2004年第18期|4775-4781|共7页
作者
Sarah K.Hirschorn; M.Joyce Dinglasan; Martin ElsnerSilvia A.ManciniGeorges Lacrampe-CouloumeElizabeth A.EdwardsBarbara Sherwood Lollar;
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作者单位

Stable Isotope Laboratory,Department of Geology,University of Toronto,Toronto,Canada M5S 3B1,Department of Chemistry,University of Toronto,Toronto,Canada M5S 3H6,and Department of Chemical Engineering and Applied Chemistry,University of Toronto,Toron;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种英语
中图分类环境化学;
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Pathway Dependent Isotopic Fractionation during Aerobic Biodegradation of 1,2-Dichloroethane

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