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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A P-IIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens
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A P-IIB-type Ca2+-ATPase is essential for stress adaptation in Physcomitrella patens

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摘要

Transient cytosolic Ca2+ (Ca2+(cyt)) elevations are early events in plant signaling pathways including those related to abiotic stress. The restoration of Ca2+ cyt to prestimulus levels involves ATP-driven Ca2+ pumps, but direct evidence for an essential role of a plant Ca(2+)ATPase in abiotic stress adaptation is missing. Here, we report on a stress-responsive Ca2+-ATPase gene (PCA1) from the moss Physcomitrella patens. Functional analysis of PCA1 in a Ca2+ transport-deficient yeast mutant suggests that PCA1 encodes a P-IIB-type Ca2+-ATPase harboring an N-terminal autoinhibitory domain. In vivo localizations identified membranes of small vacuoles as the integration site for a PCA1:GFP fusion protein. PCA1 mRNA levels are up-regulated by dehydration, NaCl, and abscisic acid, and PCA1 loss-of-function mutants (Delta PCA1) exhibit an enhanced susceptibility to salt stress. The Delta PCA1 lines show sustained elevated Ca2+(cyt) in response to salt treatment in contrast to WT that shows transient Ca2+ elevations, indicating a direct role for PCA1 in the restoration of prestimulus Ca2+(cyt). The altered Ca2+ response of the Delta PCA1 mutant lines correlates with altered expression levels of stress-induced genes, suggesting disturbance of a stress-associated signaling pathway. We propose that PCA1 is an essential component for abiotic stress adaptation in Physcomitrella involved in the generation of a specific salt-induced Ca2+ signature.

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