Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1.

McClernon DR; Vavro C; St Clair M

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Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1.

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摘要

We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.

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《Journal of Clinical Microbiology》 |2006年第6期|2280-2282|共3页
作者
McClernon DR; Vavro C; St Clair M;
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作者单位

Clinical Virology Department, GlaxoSmithKline, Research Triangle Park, NC 27709-3398, USA. daniel.r.mcclernon@gsk.com;

dfar3.dsfarm.unipd.it;

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原文格式 PDF
正文语种英语
中图分类临床医学;
关键词
assay; HIV; Base Sequence; NASBA Analysis; Pulmonary evaluation; 碱基序列;

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Evaluation of a real-time nucleic acid sequence-based amplification assay using molecular beacons for detection of human immunodeficiency virus type 1.

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