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首页> 外文期刊>Review of Scientific Instruments >Flow bioreactor design for quantitative measurements over endothelial cells using micro-particle image velocimetry
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Flow bioreactor design for quantitative measurements over endothelial cells using micro-particle image velocimetry

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摘要

Mechanotransduction in endothelial cells (ECs) is a highly complex process through which cells respond to changes in hemodynamic loading by generating biochemical signals involving gene and protein expression. To study the effects of mechanical loading on ECs in a controlled fashion, different in vitro devices have been designed to simulate or replicate various aspects of these physiological phenomena. This paper describes the design, use, and validation of a flow chamber which allows for spatially and temporally resolved micro-particle image velocimetry measurements of endothelial surface topography and stresses over living ECs immersed in pulsatile flow. This flow chamber also allows the study of co-cultures (i.e., ECs and smooth muscle cells) and the effect of different substrates (i.e., coverslip and/or polyethylene terepthalate (PET) membrane) on cellular response. In this report, the results of steady and pulsatile flow on fixed endothelial cells seeded on PET membrane and coverslip, respectively, are presented. Surface topography of ECs is computed from multiple two-dimensional flow measurements. The distributions of shear stress and wall pressure on each individual cell are also determined and the importance of both types of stress in cell remodeling is highlighted.

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  • 来源
    《Review of Scientific Instruments》 |2013年第4期|045109-1-045109-10|共10页
  • 作者

    Chia Min Leong; Abram Voorhees; Gary B. NackmanTimothy Wei;

  • 作者单位

    Department of Mechanical, Aerospace and Nuclear Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA;

    MR Research and Development, Siemens Medical Solutions, Malvern, Pennsylvania 19355, USA;

    Division of Vascular Surgery, Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USADepartment of Mechanical and Materials Engineering, University of Nebraska, Lincoln, Nebraska 68588, USA;

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  • 原文格式 PDF
  • 正文语种 英语
  • 中图分类 仪器、仪表;
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