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首页> 外文期刊>Minerals Engineering >Bioleaching of a low-grade copper ore, linking leach chemistry and microbiology
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Bioleaching of a low-grade copper ore, linking leach chemistry and microbiology

机译：低品位铜矿的生物浸出，将浸出化学和微生物学联系起来

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Three largely-independent studies were undertaken on the same heap leach system during the period of transition from processing oxidised ores to sulfide ores: monitoring of heap solutions for microorganisms, analysis of samples from a spent heap, and column tests. Microbial cell numbers arid diversity were monitored in process water samples from the transition heap over a four-year period. Cell numbers remained low throughout, 1-30 x 10~4 cells ml~(-1), possibly reflecting growth inhibition by the high element concentrations in process water. High iron, magnesium and aluminium concentrations in spent heap pregnant leach solution (PLS) are attributed to siderite and clinochlore dissolution and would be expected to impact on microbial growth. Planktonic cell numbers in a column leachate declined rapidly by two orders of magnitude when concentrations of ferric ion and sulfate exceeded 30 and 75 g L~(-1), respectively. Nevertheless, a variety of bacterial strains closely related to Acidithiobacillus (At.) ferrooxidans. At. caldus, Leptospirillum (L.) ferriphilum, Acidimicrobium (Am.) ferrooxidans, Acidiphilium (Ap.) ayptum, an Alicycloba-ciMus-related strain and Sulfobacillus (S.) thermosulfidooxidans, and the archaeon Ferroplasma (F.) acidiph-Hum were isolated, mainly from the more acidic intermediate leach solutions (ILS). Overall, the results obtained from the use of culture-dependent and culture-independent methods of community analysis were complementary and consistent. The majority of identified genera and species were present in both the process water samples from the operating heap and the solutions and ore samples from the spent heap. In the spent heap, distinct populations dominated different sample types. Leptospirillum- and Acid-ithiobacillus-Uke strains dominated PLS samples and Leptospirillum also dominated seven of eight spent ore samples and all of the heap sediment samples, making it the primary iron(II) oxidising species.
机译：在从氧化矿石加工到硫化矿石的过渡期间，对同一堆浸系统进行了三项主要独立的研究：监测堆溶液中的微生物，分析废堆样品以及进行柱测试。在四年的时间内，对过渡堆中的过程水样品中的微生物细胞数和多样性进行了监测。在整个过程中，细胞数量一直保持较低水平，为1-30 x 10〜4个细胞ml〜（-1），这可能反映了工艺水中高浓度的元素会抑制生长。废堆浸提液（PLS）中高的铁，镁和铝浓度归因于菱铁矿和斜绿石的溶解，预计会影响微生物的生长。当铁离子和硫酸根的浓度分别超过30和75 g L〜（-1）时，渗滤液中的浮游细胞数迅速下降了两个数量级。然而，各种细菌菌株与酸性硫杆菌（At。）亚铁氧化物密切相关。在。卡尔杜斯（Caldus），铁钩端螺旋体（L.），酸性铁（Am。）的铁氧化物，酸性嗜酸菌（Ap。）的菌体，与阿昔洛巴-ciMus相关的菌株和硫代芽孢杆菌（S.）的热亚砜氧化物和古细菌亚铁（F.）分离，主要是从酸性更高的中间浸提液（ILS）中分离出来的。总体而言，使用依赖于文化和不依赖于文化的社区分析方法所获得的结果是相辅相成的。在操作堆中的过程水样品以及废堆中的溶液和矿石样品中，大多数确定的属和种都存在。在用完的堆中，不同的总体主导了不同的样本类型。钩端螺旋体和酸性-ithiobacillus-Uke菌株在PLS样品中占主导地位，而钩端螺旋体在8个废矿石样品和所有堆积沉积物样品中也占主导地位，使其成为主要的铁（II）氧化物种。

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